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首页> 外文期刊>Tropical Animal Health and Production >First report of seroprevalence and genetic characterization of avian orthoreovirus in Egypt
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First report of seroprevalence and genetic characterization of avian orthoreovirus in Egypt

机译：埃及禽矫直病毒血清升迁和遗传表征的第一报告

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摘要

Recently, the Egyptian broiler industry has experienced an increased incidence of avian reovirus (ARV) infections. However, to date, no studies have been carried out to investigate the epidemiologic status of ARV infections as well as the genetic characteristics of the currently circulating ARV strains. The present study estimates the seroprevalence of ARV infections in Alexandria, El-Behera, Giza, Kafr El-Sheikh, and Gharbia governorates, Egypt, during the period 2017-2018. A total of 150 serum samples from 15 unvaccinated broiler flocks with suspicious ARV infection were screened using a commercial enzyme-linked immunosorbent assay kit. All the tested flocks were found to be positive for ARV-specific antibodies, and the overall seropositivity rate was 80.6%. Meanwhile, 5 (33.3%) flocks were confirmed for the presence of ARV through a reverse transcription-polymerase chain reaction (RT-PCR) assay based on the sigma A-encoding gene. Phylogenetic analysis based on the nucleotide sequences of the sigma A-encoding gene revealed that the obtained ARV isolate, designated EGY1, was grouped in the S1113-like cluster of ARV and displayed 100% and 98.7% nucleotide identity with the Chinese MSO1 isolate and the S1133 vaccine strain, respectively. In addition, amino acid alignments with the S1133 vaccine strain revealed that the sigma A protein of the EGY1 isolate carried the substitutions G81S and A118V. In conclusion, the present study provides the evidence for a ubiquitous distribution of ARV infection in Egypt as well as represents a starting point for genetic characterization of the currently circulating ARV strains.
机译：最近，埃及肉鸡产业越来越多的禽类reovirus（ARV）感染的发病率。然而，迄今为止，没有进行研究以研究ARV感染的流行病学状态以及目前循环的ARV菌株的遗传特征。本研究估计在2017 - 2018年期间，亚历山大，埃尔 - Behera，Giza，Kafr El-Sheikh，Kafr El-Sheikh和Gharbia Goversatorates的ARV感染的Seroprengence。使用商业酶联免疫吸附试剂盒筛选来自15个未接种的肉鸡的150个来自15个未接种的肉鸡羊群的血清样品。发现所有测试的鸡群均为ARV特异性抗体，总血清阳性率为80.6％。同时，通过基于Sigma A编码基因的逆转录聚合酶链反应（RT-PCR）测定，确认5（33.3％）群体通过逆转录聚合酶链反应（RT-PCR）测定。基于Sigma A编码基因的核苷酸序列的系统发育分析显示，所获得的ARV分离物，指定的EGY1在ARV的S1113样群中被分组，并显示100％和98.7％的核苷酸同一性与中国MSO1分离物和其中S1133分别疫苗菌株。另外，氨基酸对准与S1133疫苗菌株揭示了ΣIGMA的蛋白质的蛋白质分离物携带替代G81s和A118V。总之，本研究提供了埃及ARV感染普遍分布的证据，以及代表目前循环抗植物菌株的遗传表征的起点。

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