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首页> 外文期刊>Tropical Animal Health and Production >Detection and genetic characterization of Pasteurella multocida from alpaca (Vicugna pacos) pneumonia cases
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Detection and genetic characterization of Pasteurella multocida from alpaca (Vicugna pacos) pneumonia cases

机译：羊驼（ViCugna Pacos）肺炎患者巴斯氏菌多型的检测和遗传表征

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摘要

Pasteurella multocida is a common constituent of upper respiratory tract microbiota but is frequently isolated of alpaca lung tissues from pulmonary infections. Despite its importance, very little is known about this bacteria at molecular level. In order to characterize P. multocida isolates, 24 isolates recovered from 46 mortal acute cases in young alpacas with suspected pneumonia were analyzed, using biochemical and molecular tests for capsule and LPS typing, virulence factors detection, and ERIC-PCR genetic diversity analysis. All the P. multocida isolates belonged to the capsular type A, LPS genotype L6 (related to serotypes 10, 11, 12, and 15), and possessed virulence factors gene toxA and tbpA. ERIC-PCR analysis revealed two electrophoretic profiles, and the majority of isolates (23/24) shared the same fingerprint, indicating strong evidence that there was a common source of infection for all the affect animals. This study revealed the detection of P. multocida type A, LPS genotype L6, and toxA+ and tbpA+ from dead young alpacas with pneumonia in Peru.
机译：Pasteurella Multocada是上呼吸道麦片群的常见组成，但经常分离出来自肺部感染的alpaca肺组织。尽管重要的是，在分子水平时，对这种细菌众所周知。为了表征P. Multocida分离物，分析了从46个致命急性病例中回收的24个分离物，所述幼儿园与疑似肺炎的幼儿园，使用生化和分子试验，用于胶囊和LPS键入，毒力因子检测和Eric-PCR遗传多样性分析。所有P.多型分离株属于囊型A，LPS基因型L6（与血清型10,11,12和15相关），并具有毒力因子基因TOXA和TBPA。 ERIC-PCR分析显示出两种电泳谱，大多数分离物（23/24）共享相同的指纹，表明所有影响动物的常见感染源存在很强的证据。该研究显示，在秘鲁的肺炎中，从死亡的幼苗中检测到多型A，LPS基因型L6和TBPA +和TBPA +。

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