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Effect of reproductive methods and GnRH administration on long-term protocol in Santa Ines ewes

机译：生殖方法与GNRH管理对圣诞老人母羊长期议定书的影响

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This study aimed to determine whether reproductive performance of ewes submitted to laparoscopic timed artificial insemination (TAI) would be similar to ante meridiem (AM)/post meridiem (PM) rule and assisted natural mating (NM), and whether GnRH may enhance the pregnancy rate in TAI. In experiment I, 191 non-lactating ewes were synchronized, then TAI was performed either 48 h after progesterone (P4) removal (TAI-48 h) or 12 h after estrus detection (AM/PM); moreover, some ewes were submitted to NM (NM) as control treatment. In experiment II, 247 non-lactating ewes were allocated in five treatments, a control (no-GnRH on protocol) and four treatments arranged in a factorial design 2 x 2. The factors were time and dose of GnRH: ewes that received either 10 mu g (TAI-10 mu g-36 h) or 25 mu g of GnRH (TAI-25 mu g-36 h) 36 h after P4 removal and ewes that received either 10 mu g (TAI-10 mu g-48 h) or 25 mu g of GnRH (TAI-25 mu g-48 h) at time of insemination, 48 h after P4 removal. In experiment I, pregnancy rate in TAI-48 h was lower (P = 0.03) than AM/PM and NM. Moreover, the probability of pregnancy in TAI-48 h was higher (P = 0.06) in ewes detected in estrus early. In experiment II, the use of GnRH in TAI protocols increased (P 0.01) pregnancy rate at synchronization, and TAI-25 mu-48 h and TAI-10 mu g-36 h treatments increased (P = 0.02) pregnancy rate compered to TAI-10 mu g-48 h. We conclude that TAI decreased pregnancy rate compered to NM and AM/PM, which may be improved by GnRH use in TAI to synchronize ovulation.
机译：本研究旨在确定提交给腹腔镜定时人工授精（TAI）的母羊的生殖性能是否类似于Ante Meridiem（AM）/ Post Meridiem（PM）规则和辅助自然交配（NM），以及GNRH是否可以增强怀孕泰国速度。在实验I中，191个非乳酸母羊同步，然后在孕酮（P4）除去（泰-48h）或12小时后进行48小时，在发情检测（AM / PM）之后;此外，一些母羊被提交给NM（NM）作为对照治疗。在实验II中，在五种治疗中分配了247个非哺乳酸母羊，一种控制（协议上的NO-GNRH）和四种处理，排列在阶乘设计2 x 2.中是时间和剂量的gnrh：ewes收到10 MU G（Tai-10μg-36h）或25μggnrh（Tai-25μg-36h）36小时后36小时，接受10μg（Tai-10μg-48h的母羊在P4去除后48小时，在授精时，25μg（Tai-25μg-48h）25μg（Tai-25μg-48h）。在实验I中，大约48小时的妊娠率比AM / PM和NM更低（P = 0.03）。此外，在ESTRUS早期检测到的母羊中，Tai-48 H怀孕的概率更高（p = 0.06）。在实验II中，在同步的妊娠率增加（P <0.01）妊娠率增加（P <0.01），大部分25 mu-48 h和Tai-10μg-36h治疗（P = 0.02）妊娠率增加到台式 - 10 mu g-48 h。我们得出结论，Tai减少孕率为NM和AM / PM，可以通过在Tai中的GNRH用于同步排卵来改善。

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