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Superovulatory response and embryo quality in Katandin ewes treated with FSH or FSH plus eCG during non-breeding season

机译:在非繁殖季节的FSH或FSH和FSH和FSH处理的Katandin EWE中的超级化反应和胚胎质量

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The aim of this study was to evaluate the effect of a co-treatment of follicle-stimulating hoiri one (FSH) plus equine chorionic gonadotrophin (eCG) on serum insulin and insulin-like growth factor 1 (IGF-1) concentrations, superovulatory response, ovulatory rate, and number and production of embryos in Katandin breed ewes during the non-breeding season. Twenty Katandin ewes were synchronized with progestagens (CIDR) and assigned to two superovulation treatments (n = 10): (1): ewes treated with 200 mg ewe 1 of FSH from day 5 to 8 after CIDR insertion at decreasing doses every 12 h (FSH group) and (2) ewes treated as FSH group plus 300 fU of eCG on day 5 after CIDR insertion (FSH + eCG group). Estrous behavior was monitored and direct mating was performed. On days 7 (CIDR insertion), 0 (CIDR withdrawal), and 7 (embryo recovery), blood samples were collected to determine serum hormone concentrations. Co-treatment with eCG (FSH group) did not affect (P > 0.05) serum hormone levels. Superovulation response, ovulation rate, recovery rate, fertilization, and number of embryos were also similar > 0.05) between treatments. Compared with FSH group, FSH + eCG ewes had lower (P 0.05) number of transferable embryos and higher (P 0.05) number of oocyte and a tendency to increase the number of degenerated embryos (P = 0.07). Overall results suggest that the administration of eCG is not beneficial either to improve the ovulatory response or the amount of transferable embryos in Katandin ewes superovulated with a protocol using progesterone and FSH at decreasing doses.
机译:本研究的目的是评估卵泡刺激的HoiRi一(FSH)加上血清胰岛素和胰岛素样生长因子1(IGF-1)浓度,超级化反应的影响在非育种季节Katandin品种母羊胚胎中胚胎的排卵率和数量和生产。二十katandin ewes与progestagens(cIDR)同步,并分配到两个超级化治疗(n = 10):(1):在每12小时下降时,用200mg eWS的FSH治疗FSH的FSH处理的母羊( FSH组)和(2)母羊在CIDR插入后第5天(FSH + ECG组)第5天被视为FSH Group Plus 300 Fu。监测原始行为并进行直接交配。在第7天(CIDR插入),0(CIDR取出)和7(胚胎恢复),收集血液样品以确定血清激素浓度。与ECG(FSH组)共同治疗不影响(P> 0.05)血清激素水平。治疗之间的超级超级响应,排卵率,回收率,施肥和胚胎数量也是如此。与FSH组相比,FSH + ECG EWE具有较低的(P <0.05)可转移胚胎和更高(P <0.05)卵母细胞数量和增加退化胚胎数的趋势(P = 0.07)。总体结果表明,ECG的给药无益于改善与使用黄体酮和FSH的方案在递减剂量上使用孕激素进行的Katandin EWE中的可转移胚胎的排卵反应或可转移胚胎的量。

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