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首页> 外文期刊>Veterinary Microbiology >Methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) among employees and in the environment of a small animal hospital
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Methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) among employees and in the environment of a small animal hospital

机译：耐甲氧西林金黄色葡萄球菌（MRSA）和耐甲氧西林的葡萄球菌，抗甲氧西林的葡萄球菌（MRSP）在员工和小动物医院的环境中

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The aim of the study was to investigate methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) among employees of a small animal hospital and the hospital environment. In total, 96 swabs from employees and 73 swabs from the clinic environment were investigated. Cation-adjusted-Mueller-Hinton broth (CAMHB) + 6.5% NaCl was used for enrichment before plating on Mueller-Hinton (MH) agar with 2% NaCl and 0.25 mg/L oxacillin. The staphylococcal species was determined using MALDI-TOF MS. The isolates were subjected to mecA-PCR, macrorestriction analysis, and antimicrobial susceptibility testing. MRSA were present in five nasal swabs of the 55 employees tested and in six environmental samples, MRSP in two employees (nasal and hand swabs, each) and in three environmental samples. All isolates harboured mecA. Susceptibility testing revealed that all but one of the isolates were multiresistant. All isolates were resistant to beta-lactams and fluoroquinolones. All but one of the isolates were resistant to macrolides and lincosamides. A single MRSA was resistant to gentamicin. All MRSP were resistant to trimethoprim/sulfamethoxazole and non-susceptible to gentamicin. One isolate was also resistant to tetracycline. Macrorestriction analysis revealed three main SmaI patterns for MRSA and two main SmaI patterns for MRSP. All environmental isolates were found in areas of high people and animal traffic, such as dog ward areas, waiting and triage rooms. The finding of indistinguishable MRSA or MRSP among employees and in the environment of the small animal hospital suggests the possibility of transfer of these bacteria between humans, animals, and the hospital environment.
机译：该研究的目的是在小动物医院和医院环境中探讨耐甲氧脲耐金黄色葡萄球菌（MRSA）和甲氧西林抗性葡萄球菌伪霉菌（MRSP）。研究总共96型拭子和临床环境的73次拭子。在用2％NaCl和0.25mg / L牛奶蛋白，阳离子调节 - 穆勒 - 六烯汤（CamHB）+ 6.5％NaCl用于富含麦克风 - 六酮（MH）琼脂之前的富集。使用MALDI-TOF MS测定葡萄球菌物种。将分离物进行MECA-PCR，大射频分析和抗微生物易感测试。 MRSA出现在55名员工的五个鼻拭子中，并在六个环境样本中，两个员工MRSP（鼻和手工拭子，每次）和三个环境样本。所有隔离物都陷入了梅尔卡。易感性测试显示，除​​了其中一个分离物是多人的。所有分离株均对β-内酰胺和氟喹诺酮类含量耐药。除了一个分离物中的所有物质对大环内酯和林膦酰胺有抗性。单个MRSA对庆大霉素具有抗性。所有MRSP均对TrimethoLim /磺胺甲恶唑耐药，对庆大霉素的不敏感。一种分离物也抵抗四环素。 MacRestriction分析显示了MRSA的三种主要SMAI模式和MRSP的两个主要SMAI模式。所有环境分离株都被发现在高人和动物交通区域，如狗病房，等待和分流室。在员工和小型动物医院环境中找到无法区分的MRSA或MRSP表明，在人类，动物和医院环境之间转移这些细菌的可能性。

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