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首页> 外文期刊>Biotechnology Progress >Cryopreservation of Human Bone Marrow-Derived Mesenchymal Stem Cells with Reduced Dimethylsulfoxide and Well-Defined Freezing Solutions
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Cryopreservation of Human Bone Marrow-Derived Mesenchymal Stem Cells with Reduced Dimethylsulfoxide and Well-Defined Freezing Solutions

机译：减少二甲基亚砜和定义良好的冷冻溶液冷冻保存人骨髓源间充质干细胞

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The aim of this study is to investigate the feasibility of using well defined, serum-free freezing solutions with a reduced level of dimethylsulfoxide (DMSO) of 7.5, 5, and 2.5% (v/ v) in the combination with polyethylene glycol (PEG) or trehalose to cryopreserve human bone marrow-derived mesenchymal stem cells (hBMSCs), a main source of stem cells for cell therapy and tissue engineering. The standard laboratory freezing protocol of around 1°C/min was used in the experiments. The efficiency of 1,2-propandiol on cryopreservation of hBMSCs was explored. We measured the post-thawing cell viability and early apoptotic behaviors, cell metabolic activities, and growth dynamics. Cell morphology and osteogenic, adipogenic and chondrogenic differentiation capability were also tested after cryopreservation. The results showed that post-thawing viability of hBMSCs in 7.5% DMSO (v/v), 2.5% PEG (w/v), and 2% bovine serum albumin (BSA) (w/v) was comparable with that obtained in conventional 10% DMSO, that is, 82.9 ± 4.3% and 82.7 ± 3.7%, respectively. In addition, 5% DMSO (v/v) with 5% PEG (w/v) and 7.5% 1,2-propandiol (v/v) with 2.5% PEG (w/v) can provide good protection to hBMSCs when 2% albumin (w/v) is present. Enhanced cell viability was observed with the addition of albumin to all tested freezing solutions.
机译：这项研究的目的是研究与聚乙二醇（PEG）结合使用定义明确的无血清冷冻溶液的可行性，该溶液的二甲亚砜（DMSO）含量降低了7.5、5和2.5％（v / v） ）或海藻糖冷冻保存人骨髓源性间充质干细胞（hBMSC），这是细胞疗法和组织工程学的干细胞的主要来源。实验中使用了约1°C / min的标准实验室冷冻方案。探索了1,2-丙二醇对hBMSCs冷冻保存的效率。我们测量了解冻后的细胞活力和早期凋亡行为，细胞代谢活性以及生长动力学。冷冻保存后，还测试了细胞形态以及成骨，成脂和成软骨分化能力。结果表明，hBMSC在7.5％DMSO（v / v），2.5％PEG（w / v）和2％牛血清白蛋白（BSA）（w / v）中的解冻后活力与常规方法相当。 DMSO为10％，分别为82.9±4.3％和82.7±3.7％。此外，当2时，5％DMSO（v / v）和5％PEG（w / v）和7.5％1,2-丙二醇（v / v）和2.5％PEG（w / v）可以为hBMSC提供良好的保护2存在％白蛋白（w / v）。通过向所有测试的冷冻溶液中添加白蛋白，观察到细胞活力增强。

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