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首页> 外文期刊>Alcohol >Variants of kappa-opioid receptor gene and mRNA in alcohol-preferring and alcohol-avoiding mice.
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Variants of kappa-opioid receptor gene and mRNA in alcohol-preferring and alcohol-avoiding mice.

机译：嗜酒精和嗜酒精的小鼠中κ阿片受体基因和mRNA的变异。

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摘要

Results of recent studies have indicated an association between voluntary alcohol intake and activities of kappa-opioid receptor systems in animal models. We assessed the possibility that genetic differences observed in alcohol preference among mouse strains are related to possible polymorphisms of the kappa-opioid receptor gene (Oprk1). We compared DNA sequences of the coding region and the promoter/regulatory region of Oprk1 among C57BL/6ByJ (B6, alcohol-preferring), BALB/cJ (alcohol-avoiding), CXBI (alcohol-avoiding), and six B6.C and B6.I Recombinant QTL Introgression (RQI) strains, which carry approximately 3% of the donor BALB/cJ genome in the background B6 genome and showed various alcohol preferences. Although there were no sequence differences in the coding region, BALB/cJ had a single nucleotide polymorphism (SNP) in the promoter region, which was not detected in other strains. The results indicate that the difference in alcohol preference between B6 and BALB/cJ is not correlated with polymorphisms of Oprk1. However, results of further studies comparing Oprk1 mRNA expression between B6 and BALB/cJ showed that Oprk1 expression is regulated differently in these strains. Also, DBA/2J mice (alcohol-avoiding) showed expression of Oprk1 mRNA subtypes (alternatively spliced) different from B6 and BALB/cJ mice. Search of the Celera Genomics database indicated that DBA/2J had several SNP sites in the promoter/regulatory regions, which might explain the different expression of Oprk1 mRNA subtypes in this strain. The strain-dependent variation in the expression of alternatively spliced genes can be a significant source of phenotypic variation of complex traits such as alcohol preference.
机译：最近的研究结果表明，动物模型中自愿饮酒与κ阿片受体系统活性之间存在关联。我们评估了小鼠品系在酒精偏好方面观察到的遗传差异与κ阿片受体基因（Oprk1）的可能多态性相关的可能性。我们比较了C57BL / 6ByJ（B6，偏爱酒精），BALB / cJ（偏爱酒精），CXBI（偏爱酒精）和六个B6.C之间，Oprk1编码区和启动子/调控区的DNA序列。 B6.I重组QTL渗入（RQI）菌株，在背景B6基因组中携带约3％的供体BALB / cJ基因组，并表现出多种酒精偏好。尽管在编码区没有序列差异，但是BALB / cJ在启动子区具有单核苷酸多态性（SNP），而在其他菌株中未检测到。结果表明，B6和BALB / cJ之间的酒精偏好差异与Oprk1的多态性无关。但是，比较B6和BALB / cJ之间的Oprk1 mRNA表达的进一步研究结果表明，在这些菌株中Oprk1表达受到不同的调节。同样，DBA / 2J小鼠（避免饮酒）表现出不同于B6和BALB / cJ小鼠的Oprk1 mRNA亚型（或者剪接）的表达。在Celera基因组学数据库中的搜索表明，DBA / 2J在启动子/调控区具有多个SNP位点，这可能解释了该菌株中Oprk1 mRNA亚型的不同表达。选择性剪接基因表达中依赖菌株的变异可能是复杂性状（如酒精偏好）的表型变异的重要来源。

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