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首页> 外文期刊>Alternatives to Animal Testing and Experimentation >In Vitro Embryotoxicity Test of Four Plasticizers by Embryonic Stem Cell Test (EST)
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In Vitro Embryotoxicity Test of Four Plasticizers by Embryonic Stem Cell Test (EST)

机译：胚胎干细胞测试（EST）对四种增塑剂的体外胚胎毒性测试

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摘要

The embryonic stem cell test (EST) is a well-known in vitro embryotoxicty test protocol. We therefore investigated the embryotoxicity of four plasticizers by the EST protocol. Three parameters (ID_(50), IC_(50)ES and IC_(50)3T3) obtained by the results were substituted for the prediction model for classification. Each embryotoxicity level of DBF, BBP and DEHA was classified into the category of "weak embryotoxicity". Only DEHP was classified as "no embryotoxicity". As we carried out these experiments, we were able to identify new weak points in the EST protocol; that is, using the high concentration solution of the four plasticizers, it is difficult to make an embryoid body (EB) from the hanging drop culture of the EST protocol. A surface-active agent should be considered when such a phenomenon occurs. When using special chemical substances, improvement of the EST method from the viewpoint of physical properties is also desired.
机译：胚胎干细胞测试（EST）是众所周知的体外胚胎毒性测试方案。因此，我们通过EST方案研究了四种增塑剂的胚胎毒性。结果获得的三个参数（ID_（50），IC_（50）ES和IC_（50）3T3）被替换为用于分类的预测模型。 DBF，BBP和DEHA的每种胚胎毒性水平都归为“弱胚胎毒性”类别。仅DEHP被分类为“无胚胎毒性”。在进行这些实验时，我们能够识别出EST协议中的新弱点。也就是说，使用四种增塑剂的高浓度溶液，很难通过EST方案的悬滴培养法制成胚状体（EB）。发生这种现象时，应考虑使用表面活性剂。当使用特殊化学物质时，从物理性质的观点来看，还期望改进EST方法。

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