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首页> 外文期刊>Zygote >What are the effects of vitamin C on sperm functional properties during direct swim-up procedure?
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What are the effects of vitamin C on sperm functional properties during direct swim-up procedure?

机译：维生素C在直接筹集过程中维生素C对精子功能性质的影响是什么？

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摘要

Direct swim-up procedure is widely used to separate the motile competent spermatozoa from the antioxidant-rich semen. Subsequently, spermatozoa become more vulnerable to reactive oxygen species (ROS) due to their cytological characteristics. The effect of vitamin C, a highly concentrated antioxidant in the semen, on direct swim-up-enriched sperm population is not fully investigated. Therefore, the aim of the present study was to assess the effect of vitamin C on sperm functional properties during direct swim-up procedure. Semen samples were collected from 22 participants. Each semen sample was divided into several aliquots. The first portion was overlaid with sperm medium without ascorbic acid (0 mu M AA). The second and third fractions were overlaid with sperm medium supplemented with 300 mu M and 600 mu M AA; respectively. After 1 h of incubation, basic sperm parameters, intracellular ROS levels, acrosome reaction, chromatin integrity, and glucose uptake were assessed. Swim-up without AA significantly increased the percentage of ROS(+) spermatozoa compared with the raw semen (P<0.01). Interestingly, swim-up with 300 mu M AA did not increase the percentage of ROS(+) sperm compared with the raw semen. In parallel, the percentage of sperm with altered chromatin integrity was significantly lower in the 300 mu M AA group compared with that in the raw semen (P<0.05). These findings suggest that supplementation of vitamin C to sperm medium could be beneficial for direct swim-up-derived spermatozoa.
机译：直接扫描程序被广泛用于将动机态度从抗氧化剂的精液分离。随后，由于它们的细胞学特征，精子变得更容易受活性氧物种（ROS）。维生素C在精液中高度浓缩的抗氧化剂在直接举办的富含富集精子群上的作用未得到充分调查。因此，本研究的目的是评估维生素C在直接筹集过程中对精子功能特性的影响。从22名参与者收集精液样品。将每个精液样品分成几个等分试样。第一部分用没有抗坏血酸的精子介质覆盖（0μmaa）。第二和第三级分覆盖有补充有300μm和600μmaa的精子介质;分别。在孵育1小时后，评估基本精子参数，细胞内RO水平，染色体反应，染色质完整性和葡萄糖摄取。没有AA的扫描显着增加了ROS（+）精子的百分比与原始精液相比（P <0.01）。有趣的是，300 mu M AA的阵容并未增加与未生精液相比的ROS（+）精子的百分比。与原始精液相比，300μmAA组中，染色质完整性改变的精子的百分比显着降低（P <0.05）。这些发现表明，将维生素C与精子培养基的补充有利于直接扫描衍生的精子。

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