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Microbial Surfaces Investigated Using Atomic Force Microscopy

机译:使用原子力显微镜研究微生物表面

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This paper is dedicated to atomic force microscopy(AFM)as a progressive tool for imaging bacterial surfaces and probing their properties.The description of the technique is complemented by the explanation of the method's artifacts typical,in particular,for the imaging of bacterial cells.Sample preparation techniques are summarized in a separate section.Special attention is paid to the differences in imaging of Gram-positive and Gram-negative bacteria.Probing of mechanical properties,including elastic modulus,fragility,and adhesion of the cell walls is emphasized.The advantages of AFM in the studies of real-time cellular dynamical processes are illustrated by the experiment with the germination of spores.
机译:本文致力于原子力显微镜(AFM),作为一种对细菌表面成像和探测其特性的先进工具。对该技术的描述辅以该方法的典型伪像的解释,尤其是对细菌细胞成像的解释。样品制备技术在单独的部分中进行了概述,尤其要注意革兰氏阳性细菌和革兰氏阴性细菌在成像方面的差异,并着重研究机械性能,包括弹性模量,易碎性和细胞壁的粘附性。孢子萌发的实验说明了AFM在实时细胞动力学过程研究中的优势。

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