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首页> 外文期刊>Dyes and Pigments >A two-photon near-infrared fluorescent probe for imaging endogenous hypochlorite in cells, tissue and living mouse
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A two-photon near-infrared fluorescent probe for imaging endogenous hypochlorite in cells, tissue and living mouse

机译：用于在细胞，组织和活小鼠中成像内源性次氯酸盐的双光子近红外荧光探针

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摘要

Hypochlorite (HClO/ClO-) plays an important role in the body's immune function system and helps destroy invading bacteria and pathogens. Therefore, real-time and visual detection of hypochlorous acid in the living body is necessary. Here, we describe the first example of a two-photon excitation near-infrared light-emitting fluorescent probe (Nil-ClO) for HClO/ClO- based on Nile Red derivatives. Nil-ClO offers a large fluorescence response near 650 nm and good selectivity to HClO/ClO- in solution. The probe showed an approximately 8-fold increase in fluorescence within 1 min when it was detected at 650 nm in the presence of 30 equivalents of HClO/ClO-. The probe was further used for the fluorescence-based detection of HClO/ClO- in cells, tissues, and mice in a liver injury model. MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) experiments showed that the probe had almost no cytotoxicity at the concentrations needed for imaging. The Nil-ClO can response to HClO/ClO- in both HeLa cells and RAW cells quickly. In tissue imaging experiments, Nil-ClO was used to detect HClO/ClO- with a significant near-infrared fluorescence response down to 100 pm of depth. Finally, the obvious fluorescence enhancement in liver injury mouse model indicated a burst of endogenously generated HClO/ClO- in mouse during in vivo imaging experiments.
机译：次氯酸盐（HCLO / CLO-）在身体的免疫功能系统中起重要作用，有助于摧毁侵袭细菌和病原体。因此，需要实时和视觉检测活体中的次氯酸。这里，我们描述了基于尼罗红衍生物的HCLO / ClO-的双光子激发近红外发光荧光探针（Nil-Clo）的第一例。 Nil-CLO在650nm附近提供大的荧光反应，并对HClO / ClO-溶液的选择性良好。探针在在30当量的HClO / ClO-存在下在650nm处检测到650nm时，在1分钟内显示荧光大约8倍。探针还用于肝损伤模型中的基于荧光的HCLO / COLO-细胞，组织和小鼠的检测。 MTT（3-（4,5-二甲基-2-噻唑基）-2,5-二苯基-2H-四溴化沸石）实验表明，探针几乎在成像所需的浓度下几乎没有细胞毒性。 NIL-CLO可以快速地反应HCLO / CLO-在HELA细胞和原始细胞中。在组织成像实验中，使用NIL-CLO检测HCLO / CLO-具有明显的近红外荧光反应至100pm的深度。最后，肝损伤小鼠模型中明显的荧光增强表明在体内成像实验中的内源性产生的HCLO / ClO-突发。

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  • 来源
    《Dyes and Pigments》 |2020年第2020期|共8页
  • 作者

    Lin Xiangpeng; Chen Yunling; Bao Luo; Wang Shoujuan; Liu Keyin; Qin Wei-dong; Kong Fangong;

  • 作者单位

    Qilu Univ Technol State Key Lab Biobased Mat &

    Green Papermaking Key Lab Pulp &

    Paper Sci &

    Technol Shandong Prov Shandong Acad Sci Minist Educ Jinan 250353 Peoples R China;

    Qilu Univ Technol State Key Lab Biobased Mat &

    Green Papermaking Key Lab Pulp &

    Paper Sci &

    Technol Shandong Prov Shandong Acad Sci Minist Educ Jinan 250353 Peoples R China;

    Qilu Univ Technol State Key Lab Biobased Mat &

    Green Papermaking Key Lab Pulp &

    Paper Sci &

    Technol Shandong Prov Shandong Acad Sci Minist Educ Jinan 250353 Peoples R China;

    Qilu Univ Technol State Key Lab Biobased Mat &

    Green Papermaking Key Lab Pulp &

    Paper Sci &

    Technol Shandong Prov Shandong Acad Sci Minist Educ Jinan 250353 Peoples R China;

    Qilu Univ Technol State Key Lab Biobased Mat &

    Green Papermaking Key Lab Pulp &

    Paper Sci &

    Technol Shandong Prov Shandong Acad Sci Minist Educ Jinan 250353 Peoples R China;

    Shandong Univ Qilu Hosp Dept Crit Care Med Jinan Shandong Peoples R China;

    Qilu Univ Technol State Key Lab Biobased Mat &

    Green Papermaking Key Lab Pulp &

    Paper Sci &

    Technol Shandong Prov Shandong Acad Sci Minist Educ Jinan 250353 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 染料及中间体工业;颜料工业;
  • 关键词

    Hypochlorite detection; Two-photon; Near infrared emission; Endogenous; In vivo imaging;

    机译：次氯酸盐检测;双光子;近红外发射;内源;在体内成像;

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