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首页> 外文期刊>Biotechnology Progress >Mixed Culture Bioconversion of 16-Dehydropregnenolone Acetate to Androsta-1,4-diene-3,17-dione: Optimization of Parameters
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Mixed Culture Bioconversion of 16-Dehydropregnenolone Acetate to Androsta-1,4-diene-3,17-dione: Optimization of Parameters

机译：乙酸16-脱氢孕烯醇酮酯到Androsta-1,4-diene-3,17-dione的混合培养生物转化：参数优化

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Bioconversion o 16-dehydropregnenolone acetate (16-DPA) to androsta-1,4-diene-3,-17-dione (ADD),an intermediate for the production of female sex hormones,by mixed culture of Paeudomonas diminuta MTCC 3361 and Comamonas acidovorane MTCC 3362 is reported.Various jphysicochemical parameters for the bioconversion of 16-DPA to ADD have been optimized in shake flask cultures.Nutrient broth inoculated with actively growing co-Culture prwved ideal for bacterial growth and bioconversion.A temperature range of 35-40 deg C was most suitable hiher or lower temperatures adversely affected the bioconversion.Dimethylformamide below 2% concentration was the most suitable carrier solvent.Mazimum conversion was recorded at 0.5 mg mL~-1 16-DPA.A pH of 5.0 yielded a peak conversion of 62 mol % in 120 h incubation period.Addition of 9alpha-hydroxylase inhibitors failed to prevent further breakdown of ADD to nonsteroidal products.16-DPA conversion in a 5 Lfermenter followed a similar trend.
机译：通过Paeudomonas diminuta MTCC 3361和Comamonas的混合培养，将16-脱氢孕烯醇酮乙酸酯（16-DPA）转化为雄激素-1,4-二烯-3，-17-二酮（ADD），后者是生产女性性激素的中间体。报道了酸果烷MTCC 3362.在摇瓶培养中优化了16-DPA转化为ADD的各种生物化学理化参数;接种了积极生长的共培养物的营养肉汤为细菌生长和生物转化提供了理想的条件。温度范围为35-40摄氏度是最合适的温度或更低的温度，对生物转化率有不利影响。浓度低于2％的二甲基甲酰胺是最合适的载体溶剂。记录的最高转化率是0.5 mg mL〜-1 16-DPA.pH值为5.0时产生的峰值转化率为在120 h的孵化期中达到62 mol％。添加9alpha-羟化酶抑制剂未能阻止ADD进一步分解为非甾体产品.5发酵罐中的16-DPA转化遵循类似趋势。

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