Library of synthetic 5 ' secondary structures to manipulate mRNA stabilityin Escherichia coli

Carrier TA.; Keasling JD.

首页> 外文期刊>Biotechnology Progress >Library of synthetic 5 ' secondary structures to manipulate mRNA stabilityin Escherichia coli

【24h】

Library of synthetic 5 ' secondary structures to manipulate mRNA stabilityin Escherichia coli

机译：合成5'二级结构文库以操纵大肠杆菌中的mRNA稳定性

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

A DNA cassette system has been developed to allow for the convenient introduction of synthetic DNA oligonucleotides between the transcription and translation start sites of a gene in order to examine the effect of 5' hairpin structure and strength on mRNA stabilization. Rationally designed synthetic DNA cassettes were introduced into the 5' untranslated region of a modified lacZ gene to form hairpins at the 5' end of the mRNA. These DNA inserts influenced mRNA half-lives over an order-of-magnitude range, with some groups of predicted structures having half-lives that showed a strong correlation with hairpin strength while half-lives for another group of predicted structures exhibited little or no dependence on this property. These results indicate the importance of 5' hairpin structure and strength in determining stabilization of Escherichia coli mRNA. This synthetic library, as well others generated using the DNA cassette system described here, should prove useful in understanding the mechanisms of mRNA stabilization and in designing structures for recombinant gene expression control.

机译：已经开发了DNA盒系统，以允许在基因的转录和翻译起始位点之间方便地引入合成DNA寡核苷酸，以便检查5'发夹结构和强度对mRNA稳定的影响。将合理设计的合成DNA盒引入修饰的lacZ基因的5'非翻译区，以在mRNA的5'末端形成发夹。这些DNA插入片段在一个数量级范围内影响了mRNA的半衰期，一些预测结构的半衰期与发夹强度具有很强的相关性，而另一组预测结构的半衰期则表现出很少或没有依赖性。在此属性上。这些结果表明5'发夹结构和强度在确定大肠杆菌mRNA稳定中的重要性。该合成文库以及其他使用此处描述的DNA盒系统生成的文库，应被证明对理解mRNA稳定机制和设计重组基因表达控制结构有用。

著录项

来源
《Biotechnology Progress》 |1999年第1期|共7页
作者
Carrier TA.; Keasling JD.;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类生物工程学（生物技术）;分子生物学;
关键词
Messenger rna stability; Stem loop structure; Growth rate; Untranslated; region; Decay; Expression; Cleavage;

机译：Messenger rna稳定性;茎环结构;生长速率;未翻译;区域;衰变;表达;裂解;

相似文献

外文文献
中文文献
专利

1. Library of synthetic 5 ' secondary structures to manipulate mRNA stabilityin Escherichia coli [J] . Carrier TA., Keasling JD. Biotechnology Progress . 1999,第1期

机译：合成5'二级结构文库以操纵大肠杆菌中的mRNA稳定性
2. The secondary structure of mRNAs from Escherichia coli: its possible role in increasing the accuracy of translation [J] . Eugene G. Shpaer Nucleic acids research . 1985,第1期

机译：大肠杆菌mRNA的二级结构：其在提高翻译准确性中的可能作用
3. Conservation of mRNA secondary structures may filter out mutations in Escherichia coli evolution [J] . Chursov Andrey, Frishman Dmitrij, Shneider Alexander Nucleic Acids Research . 2013,第16期

机译：mRNA二级结构的保守性可能会滤除大肠杆菌进化过程中的突变
4. The stationary phase response regulator SprE (RssB) is involved in the regulation of polyadenylation and mRNA stability in Escherichia coli: in vivo evidence for PAP I interaction with the degradosome [C] . Valerie J. Carabetta, Thomas J. Silhavy, Ileana M. Cristea American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2009

机译：静止阶段响应调节器SPRE（RSSB）参与了大肠杆菌中的聚腺苷酸化和mRNA稳定性的调节：在体内证据表明PAP I与降解组合相互作用
5. Membrane protein secondary structure and spectral assignments by solid state NMR: Streptomyces lividans KcsA potassium channel and Escherichia coli ATP synthase subunit c. [D] . Varga, Krisztina. 2005

机译：固态NMR膜蛋白的二级结构和光谱分配：链霉菌Lividans KcsA钾通道和大肠杆菌ATP合酶亚基c。
6. Mutations Altering the Predicted Secondary Structure of a Chloroplast 5′ Untranslated Region Affect Its Physical and Biochemical Properties as Well as Its Ability To Promote Translation of Reporter mRNAs Both in the Chlamydomonas reinhardtii Chloroplast and in Escherichia coli [O] . David C. Fargo, John E. Boynton, Nicholas W. Gillham 1999

机译：改变叶绿体5非翻译区的预测二级结构的突变影响其物理和生化特性以及促进莱茵衣藻和大肠杆菌中报道基因mRNA翻译的能力
7. Mutations Altering the Predicted Secondary Structure of a Chloroplast 5′ Untranslated Region Affect Its Physical and Biochemical Properties as Well as Its Ability To Promote Translation of Reporter mRNAs Both in the Chlamydomonas reinhardtii Chloroplast and in Escherichia coli [O] . David C. Fargo, John E. Boynton, Nicholas W. Gillham 1999

机译：改变叶绿体5'未翻译区的预测二次结构的突变影响其物理和生化特性，以及其在衣原体中促进报告者MRNA翻译的能力，无论是在衣原体中的芦荟体和大肠杆菌

Library of synthetic 5 ' secondary structures to manipulate mRNA stabilityin Escherichia coli

摘要

著录项

相似文献

相关主题

期刊订阅