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首页> 外文期刊>Journal of biochemical and molecular toxicology >Isoflavone genistein inhibits estrogen‐induced chloride and bicarbonate secretory mechanisms in the uterus in rats
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Isoflavone genistein inhibits estrogen‐induced chloride and bicarbonate secretory mechanisms in the uterus in rats

机译：异黄酮Genistein抑制雌激素诱导的大鼠子宫中的氯化物和碳酸氢盐分泌机制

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Abstract We hypothesized that genistein could affect the chloride (Cl ? ) and bicarbonate (HCO 3 ? ) secretory mechanisms in uterus. Ovariectomized female rats were given estradiol or estradiol plus progesterone with 25, 50, or 100?mg/kg/day genistein. Following completion of the treatment, uterine fluid Cl ? and HCO 3 ? concentrations were determined by in vivo uterine perfusion. Uteri were subjected for molecular biological analysis (Western blot, qPCR, and immunohistochemistry) to detect levels of expression of Cystic Fibrosis transmembrane regulator (CFTR), Cl ? /HCO 3 ? exchanger (SLC26a6), Na + /HCO 3 ? cotransporter (SLC4a4), and estrogen receptor (ER)‐α and β. Coadministration of genistein resulted in decrease in Cl ? and HCO 3 ? concentrations and expression of CFTR, SLC26a6, SLC4a4, and ER‐α and ER‐β in the uteri of estradiol‐treated rats. In estradiol plus progesterone‐treated rats, a significant increase in the above parameters were observed following high‐dose genistein treatment except for the SLC24a4 level. In conclusion, genistein‐induced changes in the uterus could affect the reproductive processes that might result in infertility.
机译：摘要我们假设Genistein可以影响子宫中的氯化物（Cl→）和碳酸氢盐（HCO 3？）分泌机制。卵巢切除术术大鼠雌二醇或雌二醇加上孕酮，具有25,50或100μmg/ kg /天的核酸丁。完成治疗后，子宫液Cl？和HCO 3？通过体内子宫灌注测定浓度。 uteri进行分子生物学分析（Western印迹，QPCR和免疫组化）以检测囊性纤维化跨膜调节剂（CFTR）的表达水平，CL？ / HCO 3？交换器（SLC26A6），NA + / HCO 3？ COTORANSPORTER（SLC4A4）和雌激素受体（ER）-α和β。 CL的CODMININTRATION导致CL的降低和HCO 3？ CFTR，SLC26A6，SLC4A4和ER-α和ER-α和ER-β在雌二醇处理的大鼠中的浓度和表达。在雌二醇加上孕酮处理的大鼠中，在除了SLC24A4水平之外，在高剂量的Genistein处理之外观察到上述参数的显着增加。总之，子宫内毒性诱导的子宫内的变化可能影响可能导致不孕症的生殖过程。

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