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首页> 外文期刊>Journal of Bioscience and Bioengineering >Enhancement of the cultivation process conditions of mixed culture methanotrophic Proteobacteria phylum enriched from waste activated sludge as the first step for value added recovery process
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Enhancement of the cultivation process conditions of mixed culture methanotrophic Proteobacteria phylum enriched from waste activated sludge as the first step for value added recovery process

机译：增强混合培养培养过程条件的培养培养型植物植物植物富集废物活性污泥富集的第一步是增值恢复过程

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Methanotrophs are of great interest due to their distinguish ability of recovering value-added commodities such as methanol and lipids while mitigating methane. The enhancement of methanotrophs cultivation process conditions is a pivotal step to develop a feasible methanotrophic bioreactor. In this study, multiple batch tests have been performed to evaluate the aqueous growth medium elements including nitrogen, copper, and biomass density and the gaseous headspace composition influence on methanotrophs activity and the associated microbial community. It was found that increasing copper concentration to 20 mu M and nitrate concentration to 40 mM result in higher growth rate and yield. In contrast, increasing the biomass density resulted in a declination in the growth rate and yield. The 16S rRNA gene sequencing shows that the used culture was dominated by type I Methylomonas genus at relative abundance of 60.9% compare to 1% of the enriched population for type II and type III methanotrophs. Thereafter, the specific nitrate uptake rate has been determined to be ranging from 0.05 to 0.62 mgN-NO3/mgTSS/day based on the cultures OD600. The attained results would facilitate the continuous cultivation of type I methanotrophs dominated culture as a first step for any methanotrophic based biotechnology. (C) 2018, The Society for Biotechnology, Japan. All rights reserved.
机译：由于它们的区分能力在减轻甲烷时恢复增值商品如甲醇和脂质等增值商品的能力，但甲胰剂具有很大的兴趣。甲蛋白培养过程条件的增强是开发可行的甲基营养生物反应器的枢轴步骤。在该研究中，已经进行了多种分批测试以评估包括氮，铜和生物质密度的含水生长培养基元素和气态顶部空间成分对甲蛋白酶活性和相关的微生物群落的影响。发现将铜浓度增加至20μm，硝酸盐浓度至40mm，导致较高的生长速率和产率。相反，增加生物质密度导致生长速率和产率下降。 16S rRNA基因测序表明，使用的培养物由I型甲基胺属属地以60.9％的相对丰度与II型和III型甲蛋白酶的1％相比。此后，基于培养物OD600，确定了特定的硝酸盐摄取率为0.05至0.62mgN-no3 / mgTs /天。达到的结果将促进I型甲蛋白占型培养物的连续培养，作为任何基于甲脂蛋白的生物技术的第一步。 （c）2018年，日本生物技术协会。版权所有。

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