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首页> 外文期刊>AIChE Journal >Three-dimensional hydrogel encapsulated embryonic stem and carcinoma cells as culture platforms for cytotoxicity studies
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Three-dimensional hydrogel encapsulated embryonic stem and carcinoma cells as culture platforms for cytotoxicity studies

机译：三维水凝胶封装的胚胎干细胞和癌细胞作为细胞毒性研究的培养平台

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The utility of alginate hydrogels for three-dimensional (3-D) culture of mouse embryonic stem cells (mESCs) and future development of 3-D stem cell culture-based in vitro screens of toxicity is described. Using alginate hydrogels of various stiffness, we first evaluated the impact of substrate modulus on mESC viability, proliferation, as well as expression of pluripotency and germ-layer markers and observed that low concentration alginate hydrogels (0.5% and 1% alginate) were most suitable for long-term culture of mESCs. These results were not unique to mESCs; long-term viability and proliferation of mouse embryonic carcinoma cells (mECCs) was also best supported by similar conditions. Finally, we determined cytotoxic responses of alginate encapsulated cells to commercially available chemicals and interestingly observed similar responses for mESCs and mECCs, thereby suggesting that mECCs can predict stem cell responses to chemicals. These studies will facilitate future design of optimal stem cell-based platforms of organ-specific and developmental toxicity. (c) 2015 American Institute of Chemical Engineers AIChE J, 61: 3180-3184, 2015
机译：描述了藻酸盐水凝胶在小鼠胚胎干细胞（mESCs）的三维（3-D）培养中的实用性以及基于3-D干细胞培养物的体外毒性筛选的未来发展。我们使用各种硬度的藻酸盐水凝胶，首先评估了底物模量对mESC活力，增殖以及多能性和胚层标记物表达的影响，并观察到低浓度藻酸盐水凝胶（0.5％和1％藻酸盐）最合适用于mESC的长期培养。这些结果并不是mESC独有的。相似条件也可以最好地支持小鼠胚胎癌细胞（mECCs）的长期生存能力和增殖。最后，我们确定了藻酸盐包封的细胞对市售化学品的细胞毒性反应，并有趣地观察到了mESCs和mECCs的相似反应，从而表明mECCs可以预测干细胞对化学品的反应。这些研究将促进器官特异性和发育毒性的最佳基于干细胞的平台的未来设计。 （c）2015年美国化学工程师学会AIChE J，61：3180-3184，2015

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