• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Biofilm formation and antimicrobial susceptibility of staphylococci and enterococci from osteomyelitis associated with percutaneous orthopaedic implants
【24h】

Biofilm formation and antimicrobial susceptibility of staphylococci and enterococci from osteomyelitis associated with percutaneous orthopaedic implants

机译：葡萄球菌和肠球菌的生物膜形成和抗微生物敏感性与经皮整形植入物相关的骨髓炎

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract Staphylococci and enterococci account for most deep infections associated with bone‐anchored percutaneous implants for amputation treatment. Implant‐associated infections are difficult to treat; therefore, it is important to investigate if these infections have a biofilm origin and to determine the biofilm antimicrobial susceptibility to improve treatment strategies. The aims were: (i) to test a novel combination of the Calgary biofilm device and a custom‐made susceptibility MIC plate (Sensititre ? ), (ii) to determine the biofilm formation and antimicrobial resistance in clinical isolates causing implant‐associated osteomyelitis, and (iii) to describe the associated clinical outcome. Enterococci and staphylococci were characterized by microtitre plate assay, Congo Red Agar plate test, and PCR. Biofilm susceptibility to 10 antimicrobials and its relationship to treatment outcomes were determined. The majority of the strains produced biofilm in vitro showing inter‐ and intraspecies differences. Biofilms showed a significantly increased antimicrobial resistance compared with their planktonic counterparts. Slime‐producing strains tolerated significantly higher antimicrobial concentrations compared with non‐producers. All seven staphylococcal strains carried ica genes, but two did not produce slime. The degree of biofilm formation and up‐regulated antibiotic resistance may translate into a variable risk of treatment failure. This new method set‐up allows for the reproducible determination of minimum biofilm eradication concentration of antimicrobial agents, which may guide future antimicrobial treatment decisions in orthopaedic implant‐associated infection. ? 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2630–2640, 2017.
机译：抽象的葡萄球菌和肠球菌占与骨锚定经皮植入物相关的最深入的感染，用于截肢治疗。植入物相关的感染难以治疗;因此，重要的是调查这些感染是否具有生物膜来源，并确定改善治疗策略的生物膜抗微生物易感性。目的是：（i）测试Calgary Biofilm装置的新组合和定制的敏感性MIC板（Sigsititre？），（II），以确定临床分离株中的生物膜形成和抗微生物抗性，导致植入物相关的骨髓炎， （iii）描述相关的临床结果。肠球菌和葡萄球菌的特征在于微量滴定板测定，刚果红琼脂板试验和PCR。确定生物膜对10次抗菌药物的敏感性及其与治疗结果的关系。大多数菌株在体外产生生物膜，显示出和有内侧差异。与其浮游对应物相比，生物膜显示出显着增加的抗微生物抗性。与非生产者相比，生产粘液产量明显高的抗微生物浓度。所有七种葡萄球菌菌株都携带ICA基因，但两种没有产生粘液。生物膜形成和上调抗生素抗性的程度可以转化为治疗失败的可变风险。这种新方法设置允许可重复测定最小生物膜消除抗菌药物的抗菌药物，其可以在整形外科植入物相关感染中引导未来的抗微生物治疗决策。还2016年Wiley期刊，Inc。J生物保解员B部分B：苹果生物检索物，105B：2630-2640,2017。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号