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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Optimal dehydrothermal processing conditions to improve biocompatibility and durability of a weakly denatured collagen scaffold
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Optimal dehydrothermal processing conditions to improve biocompatibility and durability of a weakly denatured collagen scaffold

机译：最佳的脱水热处理条件，提高生物相容性和弱变性胶原屑支架的生物相容性和耐久性

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Abstract Collagen scaffolds are essential for tissue regeneration; however, preprocessing of these scaffolds is necessary because of their poor mechanical properties. The aim of this study was to determine the optimal condition for preparing a collagen scaffold with biocompatibility and durability. An atelocollagen fiber suspension was made and stored at ?10°C in a container that could be cooled from the bottom to provide an orientation perpendicular to the collagen fiber and facilitate cell infiltration into the scaffold. After freeze‐drying the frozen suspension, various collagen scaffolds were made by dehydrothermal (DHT) treatment under different conditions (processing temperature: 120–160°C for 0–28 h). Sections of the obtained materials were embedded under the back skin of rats, and the thickness and biocompatibility of the residual scaffold were evaluated after 2 weeks. The number of foreign body giant cells was counted to evaluate biocompatibility. Although the residual scaffold was thick, excessive DHT treatment caused a strong foreign body reaction. Weak DHT treatment resulted in a collagen scaffold with good biocompatibility but with reduced thickness. Overall, these results showed the restricted optimal conditions to make a collagen scaffold with good biocompatibility and ability to maintain sufficient space for tissue regeneration. ? 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2301–2307, 2017.
机译：摘要胶原屑脚手架对于组织再生至关重要;然而，由于其机械性能差，因此需要预处理这些支架。本研究的目的是确定具有生物相容性和耐久性的制备胶原蛋白支架的最佳条件。制备Etelocollagen纤维悬浮液并在容器中储存在α10℃的容器中，该容器可以从底部冷却，以提供垂直于胶原纤维的取向，并促进细胞浸润到支架中。冷冻干燥后冷冻悬浮液后，通过在不同条件下脱水热（DHT）处理进行各种胶原屑（加工温度：120-160℃0-28小时）制备。将所得材料的部分嵌入大鼠背面皮肤下，并在2周后评价残留支架的厚度和生物相容性。计数异物巨细胞的数量以评估生物相容性。虽然残留的支架厚，但过量的DHT治疗引起强大的异物反应。弱DHT处理导致胶原蛋白支架具有良好的生物相容性，但厚度减小。总体而言，这些结果显示了限制的最佳条件，使胶原屑支架具有良好的生物相容性和维持足够空间进行组织再生的能力。还2016 Wiley期刊，Inc.J生物保解员B部分：Appl Biomater，105B：2301-2307，2017。

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