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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Micropatterning of reagent-free, high energy crosslinked gelatin hydrogels for bioapplications
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Micropatterning of reagent-free, high energy crosslinked gelatin hydrogels for bioapplications

机译：无试剂，高能量交联明胶水凝胶的微透镜用于生物缺陷

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摘要

Hydrogels are crosslinked polymeric gels of great interest in the field of tissue engineering, particularly as biocompatible cell or drug carriers. Reagent-free electron irradiated gelatin is simple to manufacture, inexpensive and biocompatible. Here, the potential to micropattern gelatin hydrogel surfaces during electron irradiation crosslinking was demonstrated as a promising microfabrication technique to produce thermally stable structures on highly relevant length scales for bioapplications. In the present work, grooves of 3.75 to 170 mu m width and several hundred nanometers depth were transferred onto gelatin hydrogels during electron irradiation and characterized by 3D confocal microscopy after exposure to ambient and physiological conditions. The survival and influence of these microstructures on cellular growth was further characterized using NIH 3T3 fibroblasts. Topographical modifications produced surface structures on which the cultured fibroblasts attached and responded by adapting their morphologies. This developed technique allows for simple and effective structuring of gelatin and opens up new possibilities for irradiation crosslinked hydrogels in biomedical applications in which cell attachment and contact guidance are favored. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 320-330, 2018.
机译：水凝胶是在组织工程领域的大兴趣的交联聚合物凝胶，特别是作为生物相容性细胞或药物载体。无试剂电子照射的明胶制造简单，廉价和生物相容性简单。这里，对电子照射交联期间的微型图案凋亡水凝胶表面的电位被证明是有希望的微生物制备技术，以产生对生物缺像的高度相关长度尺度的热稳定结构。在本作工作中，在电子照射期间将3.75至170μmm宽和几百纳米深度的凹槽转移到明胶水凝胶中，并在暴露于环境和生理条件后通过3D共聚焦显微镜表征。使用NIH 3T3成纤维细胞进一步表征这些微观结构对细胞生长的存活率和影响。地形修饰产生了表面结构，通过调整它们的形态来抑制培养的成纤维细胞。这种开发的技术允许明胶简单有效的结构化，并对生物医学应用中的辐射交联水凝胶开辟了新的可能性，其中有利于细胞附着和接触引导。 （c）2017年Wiley期刊，Inc。J生物保解员B部分B：苹果生物摩特，106B：320-330,2018。

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