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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Tissue-engineering acellular scaffoldsThe significant influence of physical and procedural decellularization factors
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Tissue-engineering acellular scaffoldsThe significant influence of physical and procedural decellularization factors

机译：组织工程牙细胞脚手架对物理和程序脱细胞化因子的显着影响

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The importance of decellularized medical products has significantly increased during the last years. In this paper, we evaluated the effects of selected physical and procedural decellularization (DC) factors with the aim to systematically assess their influence on DC results. 72 porcine aortic walls (AW) were divided into three groups and exposed to a DC solution for 4 h and 8 h, either continuously or in repeated cycles. The AW were rocked (90bpm), whirled (10 l/min), sonicated (120W, 45 kHz) or exposed to a combination of these treatments, followed by 10 washing cycles. Defining successful DC as removal of nuclei while keeping an intact extracellular matrix (ECM), we equalized the efficiency to the penetration depth (PD), obtained by DAPI fluorescence and H&E staining. Additionally, we performed scanning electron microscopy (SEM), Pentachrome and Picrosirius-Red staining. Results showed that significantly higher DC depths are achieved on outer compared to inner surfaces (61 +/- 7%; p0.001). Furthermore, the PD showed a high time dependency for all samples. Compared to continuous rocking, we achieved a significant increase in the DC efficiency through cyclic treatments ( approximate to 43%), whirling ( approximate to 19%) and sonication ( approximate to 49%). The combined treatment supported these results. In all procedures, a skeletonized but intact Collagen fibrous network was obtained as confirmed by SEM analysis. In conclusion, we systematically identified essential factors to significantly enhance DC procedures. We highly recommend considering these factors in future DC protocols. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 153-162, 2018.
机译：在过去几年中，脱细胞化医疗产品的重要性大幅增加。在本文中，我们评估了所选物理和程序脱细胞化（DC）因素的影响，以系统地评估其对直流结果的影响。将72个猪主动脉壁（AW）分成三组并暴露于直流溶液4小时和8小时，连续或重复循环。摇晃（90bpm），旋转（10升/分钟），超声处理（120w，45 kHz）或暴露于这些处理的组合，然后是10个洗涤循环。定义成功的DC作为保持核的除去，同时保持完整的细胞外基质（ECM），我们将效率与DAPI荧光和H＆E染色等均衡到渗透深度（Pd）。另外，我们进行了扫描电子显微镜（SEM），五辐射和PICROSIRIUS-RED染色。结果表明，与内表面相比，在外表面（61 +/- 7％; P <0.001）外，在外部实现了显着更高的DC深度。此外，PD显示了所有样本的高时间依赖性。与连续摇摆相比，我们通过循环处理（近似为43％），旋转（近似为19％）和超声处理（近似为49％），实现了直流效率的显着增加。组合治疗支持这些结果。在所有程序中，获得骨骼化但完整的胶原纤维网络，如SEM分析证实。总之，我们系统地确定了重要因素，以显着增强直流程序。我们强烈推荐考虑未来的DC协议中的这些因素。 （c）2016 Wiley期刊，Inc .J生物保解员B部分B：Appl Biomater，106B：153-162,2018。

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