Optimized performance of the integrated hepatic cell-loaded cryogel-based bioreactor with intermittent perfusion of acute liver failure plasma

Damania Apeksha; Kumar Anupam; Sarin Shiv K.; Kumar Ashok

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Optimized performance of the integrated hepatic cell-loaded cryogel-based bioreactor with intermittent perfusion of acute liver failure plasma

机译：具有间歇灌注急性肝功能衰竭等离子体的集成肝细胞负载良好的基于冷冻凝胶的生物反应器的优化性能

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Acute liver failure (ALF) plasma has cytotoxic effects on the cell-loaded bioreactor in bioartificial liver support systems due to the presence of innumerable hepatotoxic compounds that adversely affect the morphology and functionality of the cells. We have designed a hybrid bioreactor that integrates a hepatic cell-loaded cryogel disc and an activated carbon cloth in one compact unit, with potential application as a bioartificial liver support. In this article, we assess the performance of this integrated hybrid cryogel-based bioreactor in a perfusion-based culture system and analyze its functionality and longevity in the presence of intermittent exposure to ALF plasma. The bioreactor maintained functionality in terms of glucose consumption and albumin synthesis for up to 40 days under perfusion. Additionally, intermittent perfusion of plasma from rodent models of ALF resulted in a decrease in viability and functionality only after the second spike of plasma, with the bioreactor maintaining its functionality even after the first spike. Similar results were obtained with patient plasma indicating the potential to reuse the bioreactor for multiple sessions of liver dialysis. Collectively, these results suggest the potential of the integrated cryogel-based bioreactor to be used at most twice before being disposed of. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 259-269, 2018.

机译：由于存在无数肝毒性化合物，急性肝功能衰竭（ALF）等离子体对生物肝脏支持系统中的细胞加载的生物反应器具有细胞毒性作用。对细胞的形态和功能的存在不可属的肝毒性化合物。我们设计了一种杂交生物反应器，其在一个紧凑的单元中将肝脏电池负载的冷却盘和活性炭布集成，具有潜在的应用作为生物肝脏载体。在本文中，我们评估了基于灌注的培养系统中这种基于杂种Cryogel的生物反应器的性能，并在间歇暴露于ALF等离子体的情况下分析其功能和寿命。在灌注下，生物反应器在葡萄糖消耗和白蛋白合成方面保持函数，在灌注下长达40天。另外，仅在ALF的啮齿动物模型中间歇地灌注血浆，导致在等离子体的第二穗之后的活力和功能下降，即使在第一秒筒之后也保持其功能。患者血浆获得了类似的结果，该血浆表明能够重复使用生物反应器以用于肝脏透析的多个会话。总的来说，这些结果表明，在处理之前，最多两次使用的基于CryoGel基生物反应器的潜力。（c）2017年Wiley期刊，Inc。J生物保解员B：Appl Biomater，106B：259-269,2018。

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《Journal of biomedical materials research. Part B, Applied biomaterials.》 |2018年第1期|共11页
作者
Damania Apeksha; Kumar Anupam; Sarin Shiv K.; Kumar Ashok;
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作者单位

Indian Inst Technol Kanpur Dept Biol Sci &

Bioengn Kanpur 208016 Uttar Pradesh India;

ILBS New Delhi India;

ILBS New Delhi India;

Indian Inst Technol Kanpur Dept Biol Sci &

Bioengn Kanpur 208016 Uttar Pradesh India;

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正文语种 eng
中图分类医用一般科学;
关键词
bioartificial liver device; cryogel; integrated hybrid bioreactor; acute liver failure; plasma;

机译：生物肝脏设备;冷冻凝胶;集成杂交生物反应器;急性肝功能衰竭;血浆;

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1. Optimized performance of the integrated hepatic cell-loaded cryogel-based bioreactor with intermittent perfusion of acute liver failure plasma [J] . Damania Apeksha, Kumar Anupam, Sarin Shiv K., Journal of biomedical materials research. Part B, Applied biomaterials. . 2018,第1期

机译：具有间歇灌注急性肝功能衰竭等离子体的集成肝细胞负载良好的基于冷冻凝胶的生物反应器的优化性能
2. Plasma perfusion combined with plasma exchange in chronic hepatitis B-related acute-on-chronic liver failure patients [J] . Yang Zhongyuan, Zhang Zhongwei, Cheng Qiuyu, Hepatology international . 2020,第4期

机译：血浆灌注联合慢性乙型肝炎相关急性对慢性肝功能衰竭患者的血浆交换
3. Treatment of acute hepatic failure and encephalopathy with extracorporeal ex vivo pig-liver perfusion in the critical care unit [J] . MC DaSilva, M Gupta, MJ Holman, Critical care : . 1999,第Suppla1期

机译：重症监护室采用体外离体猪肝灌注治疗急性肝衰竭和脑病
4. Influence of hepatic perfusion and application mode in an animalexperimental study on laser-induced thermotherapy (LITT) in the liver, [C] . Dirk Albrecht, M.D., Benjamin Franklin Medical Ctr./Freie Univ. Berlin, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems VII . 1997

机译：肝灌注和应用模式对激光诱导肝热疗法（LITT）的动物实验研究的影响，
5. Two formulations of the industrial surfactant, Toximul, differentially reduce hepatic glycogen in a surfactant/influenza B virus mouse model of acute liver failure: Correlations with effects on HepG2 glycogen content. [D] . Al-Khalidi, Mustafa. 2005

机译：在急性肝衰竭的表面活性剂/ B型流感病毒小鼠模型中，两种工业表面活性剂Toximul可以差异地减少肝糖原：与HepG2糖原含量影响的相关性。
6. Treatment of acute hepatic failure and encephalopathy with extracorporeal ex vivo pig-liver perfusion in the critical care unit [O] . MC DaSilva, M Gupta, MJ Holman, 1999

机译：重症监护室采用体外离体猪肝灌注治疗急性肝衰竭和脑病
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Optimized performance of the integrated hepatic cell-loaded cryogel-based bioreactor with intermittent perfusion of acute liver failure plasma

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