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首页> 外文期刊>Journal of drug delivery science and technology >In-vitro and in-vivo comparison of rSAG1-loaded PLGA prepared by encapsulation and adsorption methods as an efficient vaccine against Toxoplasma gondii ”
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In-vitro and in-vivo comparison of rSAG1-loaded PLGA prepared by encapsulation and adsorption methods as an efficient vaccine against Toxoplasma gondii ”

机译：通过封装和吸附方法制备的rsag1负载PLGA的体外和体内比较，作为针对Toxoplasma Gondii的有效疫苗“

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摘要

The emergence of delivery vehicles has revolutionized vaccine development. Although vaccination is the most effective approach to eradicate toxoplasmosis, there is nonetheless no commercially available human vaccine againstToxoplasma gondii. Here, we applied polydl-lactide-co-glycolide (PLGA) nanoparticles loaded by recombinant surface antigen 1 (rSAG1) as vaccine delivery platform. In order to assess the influence of loading method on nanoparticle immunogenicity, rSAG1-PLGA nanoparticles were prepared by both adsorption and encapsulation approaches. BALB/c mice were subcutaneously vaccinated twice with a 3-week interval by rSAG1-encapsulated PLGA, rSAG1-adsorbed PLGA, two control groups received rSAG1 and PLGA and one group remained unvaccinated. Nanoparticles characterization, rSAG1 release, cellular and humoral immune responses and the survival time after challenge withT.gondiiRH tachyzoite were investigated. Mice vaccinated with both rSAG1- PLGA nanoparticles elicited higher IFN-γ, specific anti-T.gondiiIgG antibodies and longer survival time than controls. However, rSAG1-adsorbed PLGA stimulated significantly higher amount of IFN-γ and IgG2a titers compared to rSAG1-encapsulated PLGA. This study suggests adsorption as a more appropriate approach than encapsulation in antigen loading on PLGA as vaccine vehicle. It is the first report that the effects of preparation method of PLGA nanoparticles loaded by identical antigen on their characteristics and immunogenicity are evaluated and compared.
机译：送货车的出现彻底改变了疫苗发展。虽然疫苗接种是根除弓形虫病的最有效的方法，但是仍然没有商业上可获得的人类疫苗反对氧基催化剂。这里，我们应用由重组表面抗原1（RSAG1）负载的聚二丙酯 - 共乙酰胺（PLGA）纳米颗粒作为疫苗输送平台。为了评估载荷方法对纳米颗粒免疫原性的影响，通过吸附和包封方法制备RSAG1-PLGA纳米颗粒。通过RSAG1封装的PLGA，RSAG1吸附的PLGA，rsag1吸附的PLGA，接受RSAG1和PLGA的两个对照组，并且一组仍未接受，并且一组仍未被疫苗接种了两次的疫苗。纳米粒子表征，RSAG1释放，细胞和体液免疫应答和攻击后的存活时间进行了研究。用RSAG1-PLGA纳米粒子接种的小鼠引发了更高的IFN-γ，特异性抗T.Gondiigg抗体和比对照的更长的存活时间。然而，与RSAG1包封的PLGA相比，RSAG1吸附的PLGA刺激了IFN-γ和IgG2A滴度的显着更高。该研究表明，吸附作为比PLGA抗原作为疫苗载体的抗原载荷的封装更合适的方法。第一报告称PLGA纳米颗粒的制备方法对其特征和免疫原性加载相同抗原的制备方法的影响。

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