• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of applied microbiology >Serological and molecular evidence ofMycobacterium bovisin dairy cattle and dairy farm workers under the intensive dairy production system in Egypt
【24h】

Serological and molecular evidence ofMycobacterium bovisin dairy cattle and dairy farm workers under the intensive dairy production system in Egypt

机译：埃及密集乳制品生产系统中牛杆菌乳制牛和乳制品工人的血清和分子证据

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Aims We employed a combination of diagnostic tests including single cervical tuberculin test (SCT), rapid lateral-flow test (RT), TB-Feron, conventional PCR and culture to investigate the prevalence ofMycobacterium bovisinfections in dairy cattle under the intensive dairy production system in Egypt. Methods and Results In total, 2710 dairy cows from 11 herds in six Governorates were tested by SCT and 444 (16 center dot 4%) were TB reactors. Only 65 cows responded toM. bovisantigen by RT and TB-Feron. A postmortem examination showed that 49 (75 center dot 4%) of slaughtered cows have visible lesions. Testing of 215 out 444 SCT reactor cows by culture and PCR using blood and milk samples revealed thatM. boviswere more frequently identified in the blood (6 center dot 1-20 center dot 5%) than milk (2 center dot 3-5 center dot 6%) samples. Additionally, in this study, we investigated the prevalence and potential risk factors associated withM. bovisinfection in dairy farm workers. Overall, 100 dairy farm workers were tested using QuantiFERON-TB Gold In-Tube test to detectMycobacterium tuberculosiscomplex (MTC) and 35 (35%) were positive. In all, 23 (23%) of MTC positive wereM. bovispositive using PCR.Mycobacterium bovispositive cases were associated with workers who had respiratory signs and did not wash or disinfect their hands after handling cows. Conclusions The prevalence ofM. bovisin dairy cows and dairy farm workers under the intensive dairy production system in Egypt is high. It is therefore essential to disseminate effective prevention and control measures to prevent the spread ofM. bovisbetween dairy cows and dairy workers. Significance and Impact of the Study This study revealed that the use of RT or TB-Feron as an ancillary test of SCT reactor cows resulted in a significant reduction in the SCT false-positive slaughtered cows. A high prevalence ofM. bovisinfection among farm workers provides evidence of occupational risk in the intensive dairy production system in Egypt.
机译：目的是我们使用诊断试验的组合，包括单宫颈结核蛋白检测（SCT），快速横向 - 流量试验（RT），TB-Feron，常规PCR和培养，以研究在密集的乳制品生产系统下的乳制品中的牛奶杆菌中的细菌培养率普及埃及。通过SCT和424个左右的11个牛群的总共2710乳头奶牛的方法和结果得到了444（16个中心点4％）的TB反应器。只有65母牛应对汤姆。通过RT和TB-Feron的Bovisantigen。后期检查显示，49（75个中心4％）的屠宰牛具有可见的病变。通过培养和使用血液和牛奶样品的培养和PCR检测215次444 SCT反应器奶牛显示出来。 Boviswere更常见于血液（6中心点1-20中心点5％）比牛奶（2中心点3-5中心点6％）样品。此外，在本研究中，我们研究了与M相关的患病率和潜在的风险因素。奶牛在奶牛场工作者的植物抚养。总体而言，使用Quantiferon-TB金管试验测试了100名乳制品工人，对探测细胞核分子复用（MTC），35（35％）是阳性的。总而言之，23（23％）的MTC阳性Werem。使用PCR的Bovispositive.Mocobacterium Bovisspositive病例与患有呼吸迹象的工人有关，并且在处理奶牛后没有洗涤或消毒他们的手。结论患病率。埃及密集型乳制品生产系统下的Bovisin奶牛和乳制品工人很高。因此，必须传播有效的预防和控制措施来防止蔓延。 Bovisbetwe乳制奶牛和乳制品工人。该研究的意义和影响本研究表明，使用RT或TB-Feron作为SCT反应堆牛的辅助测试导致SCT假阳性屠宰奶牛的显着减少。高患病率。农业工人之间的植物抚育提供了埃及密集的乳制品生产系统中的职业风险证据。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号