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The development of chicken cerebellar cortex and the determination of AgNOR activity of the Purkinje cell nuclei

机译:鸡小脑皮质的发育及浦肯野细胞核AgNOR活性的测定

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摘要

In this study, the embryonic and post-hatching development of the chicken cerebellar cortex, and the number, size and area of the silver-staining nucleolus-organizer regions (AgNORs) were determined in cerebellar Purkinje cells in layer-hen (Babcock-B380) embryos and chickens. The mean transverse diameter of the Purkinje cells and their nuclei, and the mean area of the Purkinje cell nucleus and AgNOR area increased during the entire experimental period. The mean ratio of the AgNOR area to the Purkinje cell nucleus area reached its highest value at the 15th day of incubation. There was no difference between the mean AgNOR numbers per nucleus. These findings provide reference data for future studies in this and related research topics, for example for establishing AgNOR parameters and we also hope that our data will be supplemented with other techniques such as PCNA and TUNEL in the near future.
机译:在这项研究中,确定了小鸡母鸡小脑浦肯野细胞(Babcock-B380)中小脑皮质的胚胎和孵化后的发育,以及染银的核仁组织者区域(AgNORs)的数量,大小和面积。 )胚胎和鸡。在整个实验期间,浦肯野细胞及其细胞核的平均横向直径以及浦肯野细胞核的平均面积和AgNOR面积均增加。在孵育的第15天,AgNOR面积与Purkinje细胞核面积的平均比值达到最高值。每个核的平均AgNOR数之间没有差异。这些发现为该研究及相关研究主题中的未来研究提供了参考数据,例如用于建立AgNOR参数,并且我们还希望在不久的将来,我们的数据将被其他技术(例如PCNA和TUNEL)所补充。

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