• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Belgian Journal of Zoology >Salivary amino acid concentrations in zebus (Bos indicus) and zebu hybrids (Bos indicus x Bos taurus) fed a tannin-rich diet
【24h】

Salivary amino acid concentrations in zebus (Bos indicus) and zebu hybrids (Bos indicus x Bos taurus) fed a tannin-rich diet

机译：饲喂富含单宁的饮食的斑马（Bos indicus）和杂种牛（Bos indicus x Bos taurus）中唾液氨基酸的浓度

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Many animals show adaptation to tannins in the form of tannin-binding salivary proteins(1).Among ruminants,such proteins have been demonstrated in saliva of several species (usually browsers and intermediate feeders) (2,3,4,13).There is some circumstantial evidence to suggest that zebu cattle (Bos indicus) are different from temperate cattle breeds with respect to their salivary and digestive physiology. Apart from differences in susceptibility to heat and tropical disease (5),a difference in salivary anti-tannin defenses (and a resulting difference in rumen physiology) could be another reason zebu cattle are particularly suited for agricultural systems in the tropics,where available forages often contain high levels of tannins (6,7).Although non-proline-rich proteins exist that also have affinity for tannins (1, 8),it is interesting to compare the proline content of different cattle breeds.Here,we report such a screening for a comparison of zebu cattle and zebu-HolsteinFriesian in the Jimma area located at 7°40'N and 36°50'E at 1760 masl in southwest Ethiopia.For the study eight heifers were used:four were zebu (100% Bos indicus) and four were zebu× Holstein Friesian (HF) crosses.The blood level of crossbreed heifers (Bos indicus x Bos taurus) were composed of 70% HF+30% zebu, heifer 1; 66% HF+ 34% zebu,heifer 2;68% HF+ 32% zebu,heifer 3 and 70% HF+ 30% zebu,heifer 4.The animals were 2.5 years old with comparable body weight and similar body condition scores.The body condition score was evaluated based on 1-9 point score scale (9).They were fed on a diet that included the tannin-rich plant Albizia gummifera for 28 days.The animals were fed on a local hay mixture as a basal diet and experimental diet of leaves of A.gummifera.The diets were composed weekly to ensure that cattle would consume A.gummifera at a rate of 10% of total dry matter (DM) requirement,estimated as 2.5% of live body weight.To minimize selectivity by the animals,the A.gummifera forage was provided in the morning (8:00) whereas hay mixture was offered only later at 10:30.After 21 days,saliva samples were collected from the animals' mouths using a sponge. When the sponge was saturated with saliva,it was squeezed manually (with the investigator wearing fresh latex gloves),allowing the collection of a minimum of 10 ml saliva into a plastic cup with screw top. The saliva was then passed through a tea sieve to remove feed particles,and stored at -43℃. When the samples were thawed for analysis, they were passed through a 0.3μm syringe filter to remove bacteria.Amino acids were determined according to Hendriks et al.(2002).From these data,the proportion of proline in the total amount of measured amino acids was calculated.Differences between genotypes were evaluated by means of a Student's t-test.Significant differences were considered at P < 0.05.
机译：许多动物都以单宁结合唾液蛋白的形式表现出对单宁的适应性（1）。在反刍动物中，这种蛋白已在几种物种的唾液中被证实（通常是浏览器和中间饲养者）（2、3、4、13）。有一些间接证据表明，牛（Bos indicus）的唾液和消化生理与温带牛不同。除了对热和热带病的敏感性不同（5）外，唾液中抗单宁防御能力的差异（以及由此导致的瘤胃生理差异）可能是牛特别适合热带地区农业系统（可能有牧草）的另一个原因通常含有高水平的单宁酸（6,7）。尽管存在非脯氨酸丰富的蛋白质，并且对单宁酸也具有亲和力（1，8），但是比较不同品种牛的脯氨酸含量是很有趣的。在埃塞俄比亚西南部1760 masl处7°40'N和36°50'E的Jimma地区对zebu牛和zebu-HolsteinFriesian进行比较的筛选。研究使用了八头小母牛：四头为zebu（100％ Bos indicus）和四个是zebu×Holstein Friesian（HF）杂交。杂种小母牛（Bos indicus x Bos taurus）的血液水平由70％HF + 30％zebu（小母牛1）组成; 66％HF + 34％瘤牛，小母牛2; 68％HF + 32％瘤牛，小母牛3和70％HF + 30％瘤牛，小母牛4.动物年龄为2.5岁，体重相当且身体状况得分相近。根据1-9分的评分量表（9）进行评估，饲喂28日，其中包括富含单宁的植物Albizia gummifera，基础饲料和实验性饲喂动物干草。每周组成日粮，以确保牛以总干物质（DM）需求量的10％的比率食用牛肝菌，估计占活体重的2.5％。 ，早晨（8:00）提供了农杆菌草料，而干草混合物仅在10:30才提供。21天后，用海绵从动物的嘴中收集唾液样品。当海绵充满唾液后，将其手动挤压（研究人员戴着新鲜的乳胶手套），使至少10毫升的唾液收集到带螺丝顶盖的塑料杯中。然后将唾液通过茶筛以去除饲料颗粒，并储存在-43℃。将样品融化进行分析时，将其通过0.3μm的注射器过滤器以去除细菌。根据Hendriks等人（2002）的方法测定氨基酸。从这些数据中，脯氨酸在被测氨基酸总量中的比例进行了酸计算，通过学生t检验评估了基因型之间的差异，P <0.05为差异有统计学意义。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号