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首页> 外文期刊>Journal of Economic Entomology >Differentiation of European Corn Borer (Lepidoptera: Crambidae) and American Lotus Borer (Lepidoptera: Crambidae), Ostrinia penitalis, from North American Field Collections
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Differentiation of European Corn Borer (Lepidoptera: Crambidae) and American Lotus Borer (Lepidoptera: Crambidae), Ostrinia penitalis, from North American Field Collections

机译:欧洲玉米螟(Lepidoptera:Crambidae)和美国莲花螟(鳞翅目:Crambidae),Ostrinia Penitalis,来自北美野外收藏的差异化

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摘要

The European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae), is a perennial insect pest of cultivated maize that was inadvertently introduced into North America in the early 1900s, but population densities have decreased since the widespread adoption of transgenic hybrids that express Bacillus thuringiensis (Bt) toxins. The native American lotus borer, Ostrinia penitalis (Lepidoptera: Crambidae), is among the most ancestral species described in the genus Ostrinia, and has a geographic range that coincides with that of O. nubilalis across major maize growing regions of North America. Due to the recent decrease in O. nubilalis populations, O. penitalis has become more pronounced in light trap samples intended to monitor O. nubilalis. A molecular tool based on variation in restriction endonuclease digestion pattern of a polymerase chain reaction amplified fragment of the mitochondrial cytochrome c oxidase subunit I (coxI) gene was developed and validated to differentiate these two species. This method was applied to light trap samples over a 2-yr period and achieved accurate quantification of species, and shows that O. penitalis can be prevalent in O. nubilalis first flight sampling. These methods are useful for contemporary O. nubilalis field research in North America.
机译:欧洲玉米螟,鸵肽·纳米尔(Lepidoptera:Crambidae)是一种常年虫害的栽培玉米植物在20世纪初无意中引入北美,但由于表达芽孢杆菌的转基因杂种的广泛采用,人群密度降低了( BT)毒素。美国原住民莲花螟,鸵肽Penitalis(Lepidoptera:Crambidae)是蛇核苷属中描述的最多祖先物种之一,并且具有与北美主要玉米种植区的O. Nubilalis的地理范围。由于近期O. Nubilalis种群的减少,O.Penitalis在旨在监测O. Nubilalis的光陷阱样品中变得更加明显。显着基于线粒体链反应的限制性内切核酸酶消化模式的分子工具,并开发了线粒体链胞嘧啶C氧化酶亚基I(COXI)基因的分子蛋白的分子,并验证以区分这两个物种。将该方法应用于2年期间的光阱样品,并达到了精确定量的物种,并表明O. Penitalis在O. Nubilalis首次飞行采样中可以普遍存在。这些方法可用于北美的当代O. Nubilalis野外研究。

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