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首页> 外文期刊>Journal of Endodontics: Official Journal of American Association of Endodontists >Evaluation of a Commercially Available Hyaluronic Acid Hydrogel (Restylane) as Injectable Scaffold for Dental Pulp Regeneration: An In Vitro Evaluation
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Evaluation of a Commercially Available Hyaluronic Acid Hydrogel (Restylane) as Injectable Scaffold for Dental Pulp Regeneration: An In Vitro Evaluation

机译：商业上可获得的透明质酸水凝胶（RESTYLANE）作为牙髓再生的可注射支架的评价：体外评价

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Introduction: Regenerative endodontic procedures (REPs) are viable alternatives for treating immature teeth, yet these procedures do not predictably lead to pulp-dentin regeneration. A true bioengineering approach for dental pulp regeneration requires the incorporation of a scaffold conducive with the regeneration of the pulp-dentin complex. Several materials have been proposed as scaffolds for REPs; nonetheless, the majority are not eligible for immediate clinical chairside use. Thus, the aim of this study was to evaluate Restylane, a Food and Drug Administration-approved hyaluronic acid based gel, as possible scaffold for REPs. Methods: Stem cells of the apical papilla (SCAP) were cultured either alone or in mixtures with either Restylane or Matrigel scaffolds. Groups were cultured in basal culture medium for 6, 24, and 72 hours, and cell viability was assessed. For the mineralizing differentiation experiments, groups were cultured in differentiation medium either for 7 days and processed for alkaline phosphatase activity or for 14 days and processed for gene expression by using quantitative reverse-transcription polymerase chain reaction. SCAP in basal medium served as control. Results: Cell encapsulation in either Restylane or Matrigel demonstrated reduced cell viability compared with control. Nonetheless, cell viability significantly increased in the Restylane group in the course of 3 days, whereas it decreased significantly" in the Matrigel group. Restylane promoted significantly greater alkaline phosphatase activity and upregulation of dentin sialophosphoprotein, dentin matrix acidic phosphoprotein-1, and matrix extracellular phosphoglycoprotein, compared with control. Conclusions: A Food and Drug Administration-approved hyaluronic acid based injectable gel promoted SCAP survival, mineralization, and differentiation into an odontoblastic phenotype and may be a promising scaffold material for REPs.
机译：介绍：再生牙髓手术（REPS）是治疗未成熟牙齿的可行替代品，但这些程序不会预测可预测可导致纸浆牙本质再生。真正的牙齿浆再生的生物工程方法需要掺入有利于纸浆牙本质复合物的再生的支架。已经提出了几种材料作为代表的支架;尽管如此，大多数人没有资格获得立即临床主席使用。因此，本研究的目的是评估Reasylane，食品和药物管理批准的透明质酸的凝胶，作为代表的可能支架。方法：具有依赖性亚替纳或基质支架的混合物，培养顶乳乳头（SCAP）的干细胞。基团在基础培养基中培养6,24和72小时，并评估细胞活力。对于矿化分化实验，将基团在分化培养基中培养7天，并通过使用定量的逆转录聚合酶链反应处理碱性磷酸酶活性或14天，并加工基因表达。基础培养基中的垫片用作控制。结果：与对照相比，依赖性含量或基质胶中的细胞包封证明了细胞活力降低。尽管如此，在3天的过程中，细胞活力明显增加，而在3天内，它在基质基团中显着下降。Reyylane促进了明显更大的碱性磷酸酶活性和牙本质基质酸性磷蛋白-1和基质的牙齿磷酸磷酸酯活性和上调性磷蛋白质与对照相比。结论：食品和药物管理批准的透明质酸的可注射凝胶促进了SCAP存活，矿化和分化为Odontobolic表型，并且可以是用于代表的有前途的支架材料。

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