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首页> 外文期刊>Journal of Experimental Botany >Functional inactivation of UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) induces early leaf senescence and defence responses in rice
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Functional inactivation of UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) induces early leaf senescence and defence responses in rice

机译：UDP-N-乙酰葡糖胺酸化酶1（UAP1）的功能灭活诱导水稻中早期叶片衰老和防御反应

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摘要

Plant leaf senescence and defence responses are important biological processes, but the molecular mechanisms involved are not well understood. This study identified a new rice mutant, spotted leaf 29 (spl29). The SPL29 gene was identified by map-based cloning, and SPL29 was confirmed as UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) by enzymatic analysis. The mutant spl29 lacks UAP activity. The biological phenotypes for which UAP is responsible have not previously been reported in plants. The spl29 mutant displayed early leaf senescence, confirmed by chlorophyll loss and photosystem II decline as physiological indicators, chloroplast degradation as a cellular characteristic, and both upregulation of senescence transcription factors and senescence-associated genes, and downregulation of photosynthesis-related genes, as molecular evidence. Defence responses were induced in the spl29 mutant, shown by enhanced resistance to bacterial blight inoculation and upregulation of defence response genes. Reactive oxygen species, including O-2(-) and H2O2, accumulated in spl29 plants; there was also increased malondialdehyde content. Enhanced superoxide dismutase activity combined with normal catalase activity in spl29 could be responsible for H2O2 accumulation. The plant hormones jasmonic acid and abscisic acid also accumulated in spl29 plants. ROS and plant hormones probably play important roles in early leaf senescence and defence responses in the spl29 mutant. Based on these findings, it is suggested that UAP1 is involved in regulating leaf senescence and defence responses in rice.
机译：植物叶片衰老和防御反应是重要的生物过程，但涉及的分子机制并不顺利。该研究确定了一种新的米突变体，斑点叶29（SPL29）。通过基于地图的克隆鉴定SPL29基因，通过酶分析证实了SPL29作为UDP-N-乙酰葡糖胺焦磷酸化酶1（UAP1）。突变SPL29缺乏UAP活动。先前没有在植物中报道UAP负责的生物表型。 SPL29突变体显示早期叶片衰老，通过叶绿素损失和光系统II证实作为生理指标，叶绿体降解作为细胞特征，以及衰老转录因子和衰老相关基因的上调，以及分子的下调相关基因的下调证据。在SPL29突变体中诱导防御反应，通过增强抗细菌性枯萎的接种和对抗反应基因的上调而显示。反应性氧物种，包括O-2（ - ）和H 2 O 2，积累在SPL29植物中;还有丙二醛含量增加。增强的超氧化物歧化酶活性与SPL29中的正常过缩酶活性联合可能是H 2 O 2积累的原因。植物激素茉莉酸和脱落酸也积累在SPL29植物中。 ROS和植物激素可能在SPL29突变体中发挥早期叶片衰老和防御反应的重要作用。基于这些发现，建议UAP1参与调节水稻中的叶片衰老和防御反应。

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  • 来源
    《Journal of Experimental Botany》 |2015年第3期|共15页
  • 作者

    Wang Zhaohai; Wang Ya; Hong Xiao; Hu Daoheng; Liu Caixiang; Yang Jing; Huang Yunqing; Feng Yuqi; Gong Hanyu; Li Yang; Fang Gen; Tang Huiru; Li Yangsheng;

  • 作者单位

    Wuhan Univ State Key Lab Hybrid Rice Coll Life Sci Wuhan 430072 Hubei Peoples R China;

    Wuhan Univ State Key Lab Hybrid Rice Coll Life Sci Wuhan 430072 Hubei Peoples R China;

    Wuhan Univ State Key Lab Hybrid Rice Coll Life Sci Wuhan 430072 Hubei Peoples R China;

    Wuhan Univ State Key Lab Hybrid Rice Coll Life Sci Wuhan 430072 Hubei Peoples R China;

    Chinese Acad Sci Key Lab Magnet Resonance Biol Syst State Key Lab Magnet Resonance &

    Atom &

    Mol Phys Wuhan Ctr Magnet Resonance Wuhan Inst Phys &

    Math Wuhan 430071 Peoples R China;

    Wuhan Univ State Key Lab Hybrid Rice Coll Life Sci Wuhan 430072 Hubei Peoples R China;

    Wuhan Univ Key Lab Analyt Chem Biol &

    Med Minist Educ Dept Chem Wuhan 430072 Hubei Peoples R China;

    Wuhan Univ Key Lab Analyt Chem Biol &

    Med Minist Educ Dept Chem Wuhan 430072 Hubei Peoples R China;

    Wuhan Univ State Key Lab Hybrid Rice Coll Life Sci Wuhan 430072 Hubei Peoples R China;

    Wuhan Univ State Key Lab Hybrid Rice Coll Life Sci Wuhan 430072 Hubei Peoples R China;

    Wuhan Univ State Key Lab Hybrid Rice Coll Life Sci Wuhan 430072 Hubei Peoples R China;

    Chinese Acad Sci Key Lab Magnet Resonance Biol Syst State Key Lab Magnet Resonance &

    Atom &

    Mol Phys Wuhan Ctr Magnet Resonance Wuhan Inst Phys &

    Math Wuhan 430071 Peoples R China;

    Wuhan Univ State Key Lab Hybrid Rice Coll Life Sci Wuhan 430072 Hubei Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物学;
  • 关键词

    Defence responses; leaf senescence; reactive oxygen species (ROS); rice (Oryza sativa); SPL29; UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1);

    机译：防御反应;叶衰老;反应性氧（ROS）;米（Oryza sativa）;SPL29;UDP-N-乙酰葡糖胺酸化酶1（UAP1）;

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