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首页> 外文期刊>Journal of Experimental Botany >Functional analysis of the TM6 MADS-box gene in the octoploid strawberry by CRISPR/Cas9-directed mutagenesis
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Functional analysis of the TM6 MADS-box gene in the octoploid strawberry by CRISPR/Cas9-directed mutagenesis

机译：CRISPR / CAS9导向诱变八薄草莓TM6疯箱基因的功能分析

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摘要

The B-class of MADS-box transcription factors has been studied in many plant species, but remains functionally uncharacterized in Rosaceae. APETALA3 (AP3), a member of this class, controls petal and stamen identities in Arabidopsis. In this study, we identified two members of the AP3 lineage in cultivated strawberry, Fragaria x ananassa, namely FaAP3 and FaTM6. FaTM6, and not FaAP3, showed an expression pattern equivalent to that of AP3 in Arabidopsis. We used the CRISPR/Cas9 genome editing system for the first time in an octoploid species to characterize the function of TM6 in strawberry flower development. An analysis by high-throughput sequencing of the FaTM6 locus spanning the target sites showed highly efficient genome editing already present in the T0 generation. Phenotypic characterization of the mutant lines indicated that FaTM6 plays a key role in anther development in strawberry. Our results validate the use of the CRISPR/Cas9 system for gene functional analysis in F. x ananassa as an octoploid species, and offer new opportunities for engineering strawberry to improve traits of interest in breeding programs.
机译：在许多植物物种中研究了B级疯狂箱转录因子，但在蔷薇科在功能上无表。 Apetala3（AP3），该类的成员，控制拟南芥的花瓣和雄蕊相同。在这项研究中，我们确定了AP3谱系中的两个成员在耕种的草莓，Fragaria X ananassa，即Faap3和Fatm6。 FATM6，而不是FAAP3，表达了与AP3中AP3中的表达模式相当于拟南芥。我们在八倍体物种中首次使用CRISPR / CAS9基因组编辑系统，以表征TM6在草莓花发育中的功能。跨越靶位点的FATM6基因座的高通量测序分析显示了在T0代中已经存在的高效基因组编辑。突变线的表型表征表明FATM6在草莓中的花药发育中起着关键作用。我们的结果验证了F. X ananassa中F. X ananassa的基因功能分析的使用，为八倍体种类提供了新的机会，为工程草莓提供了改善育种计划的特征。

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