• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Advances: An International Review Journal >Escherichia coli as a production host for novel enzymes from basidiomycota
【24h】

Escherichia coli as a production host for novel enzymes from basidiomycota

机译：大肠杆菌作为担子菌新酶的生产宿主

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Many enzymes from basidiomycota have been identified and more recently characterized on the molecular level. This report summarizes the potential biotechnological applications of these enzymes and evaluates recent advances in their heterologous expression in Escherichia coli. Being one of the most widely used hosts for the production of recombinant proteins, there are, however, recurrent problems of recovering substantial yields of correctly folded and active enzymes. Various strategies for the efficient production of recombinant proteins from basidiomycetous fungi are reviewed including the current knowledge on vectors and expression strains, as well as methods for enhancing the solubility of target expression products and their purification. Research efforts towards the refolding of recombinant oxidoreductases and hydrolases are presented to illustrate successful production strategies. (c) 2014 Elsevier Inc. All rights reserved.
机译：已经鉴定了来自担子菌的许多酶，并且最近在分子水平上对其进行了表征。该报告总结了这些酶的潜在生物技术应用，并评估了它们在大肠杆菌中异源表达的最新进展。然而，作为生产重组蛋白的最广泛使用的宿主之一，仍然存在反复出现的问题，即回收正确折叠的活性酶的大量产量。综述了从担子菌真菌有效生产重组蛋白的各种策略，包括有关载体和表达菌株的最新知识，以及增强靶标表达产物的溶解度及其纯化方法。提出了有关重组氧化还原酶和水解酶再折叠的研究工作，以说明成功的生产策略。 （c）2014 Elsevier Inc.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号