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首页> 外文期刊>Belgian Journal of Zoology >Hurdles in investigating UVB damage in the putative ancient asexual Darwinula stevensoni (Ostracoda, Crustacea)
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Hurdles in investigating UVB damage in the putative ancient asexual Darwinula stevensoni (Ostracoda, Crustacea)

机译：调查推测的远古无性Darwinula stevensoni（Ostracoda，甲壳纲）中UVB损伤的障碍

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摘要

Ostracoda or mussel-shrimps are small, bivalved Crustacea. Because of their excellent fossil record and their broad variety of reproductive modes, ostracods are of great interest as a model group in ecological and evolutionary research. Here, we investigated damage and repair from one of the most important biological mutagens, namely UVB radiation, in the putative ancient asexual ostracod Darwinula stevensoni from Belgium. We applied three different methods:the Polymerase Inhibition (PI) assay, Enzyme-Linked Immuno Sorbent Assay (ELISA) and dot blot. All three techniques were unsuccessful in quantifying UVB damage in D. stevensoni. Previous experiments have revealed that the valves of D. stevensoni provide an average UVB protection of approximate 60%. Thus, UVB damage could be too little to make quantitative experiments work. Additionally, variation between individual ostracods due to season and age most likely contributed further to the failure of the three used experimental approaches to quantify damage. In a second experiment, we investigated the influence of temperature on survival of D. stevensoni during UVB exposure. The estimated relative lethal UVB dose at 4°C was with 50 kJ/m2, significantly lower than at room temperature, with 130 kJ/m2. This could either indicate lack of adaptation to low temperatures and/or the presence of metabolic processes active at room temperature protecting against UVB damage in D. stevensoni. The latter possibility could also explain why the estimated relative lethal UVB dose of D. stevensoni is similar to that of other non-marine ostracods where valves provide around 80% protection, despite the valves of D. stevensoni providing less protection. If such metabolic processes can repair UVB damage quickly, this may provide an alternative explanation why we could not quantify UVB damage in D. stevensoni.
机译：梭子鱼或贻贝虾是小的双壳甲壳纲动物。由于其优良的化石记录和广泛的生殖方式，作为生态学和进化研究中的模型组，兽脚类动物引起了极大的兴趣。在这里，我们调查了最重要的生物诱变剂之一，即UVB辐射，对比利时假定的远古无性成年成龙类Darwinula stevensoni的破坏和修复作用。我们应用了三种不同的方法：聚合酶抑制（PI）测定，酶联免疫吸附测定（ELISA）和斑点印迹。三种技术都未能成功定量史蒂文尼丝菌中的UVB损伤。先前的实验表明，史蒂文森菌的阀门可提供约60％的平均UVB保护。因此，UVB损坏可能太小而无法进行定量实验。此外，由于季节和年龄的原因，各个成龙类之间的差异极有可能进一步导致了三种用于量化危害的实验方法的失败。在第二个实验中，我们研究了温度对D. stevensoni在UVB暴露期间存活的影响。在4°C时，估计的相对致死UVB剂量为50 kJ / m2，大大低于室温下的130 kJ / m2。这可能表明缺乏对低温的适应性和/或在室温下活跃的代谢过程的存在，从而防止了史蒂文氏菌中的UVB损伤。后一种可能性也可以解释为什么估计的D. stevensoni相对UVB致死剂量与其他非海洋类龙虾相似，尽管D. stevensoni的阀门提供的保护较少，但其阀门可提供约80％的保护。如果这样的代谢过程可以快速修复UVB损伤，这可能提供了另一种解释，为什么我们无法量化D. stevensoni中的UVB损伤。

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