• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Immunological Methods >A novel method for sensitive, low-cost and portable detection of hepatitis B surface antigen using a personal glucose meter
【24h】

A novel method for sensitive, low-cost and portable detection of hepatitis B surface antigen using a personal glucose meter

机译：一种使用个人葡萄糖仪的肝炎表面抗原敏感，低成本和便携式检测的新方法

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Hepatitis B virus (HBV) infection is the major public health problem leading cause of death worldwide. The most important diagnostic marker for this infection is hepatitis B surface antigen (HBsAg). In this study, a novel, inexpensive, portable and sensitive ELISA method was designed and investigated for diagnosis of HBsAg based on the functionalized Fe3O4 and Al2O3 nanoparticles, with the strategy for detecting the concentration of glucose using a cheap and accessible personal glucose meter (PGM). The ELISA system was constructed using hepatitis B antibody against HBsAg immobilized on streptavidin coated magnetic iron oxide particles (S-Fe3O4) as the capture antibody (Ab(1)). In addition, another hepatitis B antibody against different epitope of HBsAg (Ab(2)) and glucoamylase both were immobilized on Al2O3 nanoparticles. After formation of the sandwich immune complex between Ab(1) and Ab(2) immobilized on S-Fe3O4 and Al2O3 NPs, respectively, through HBsAg, starch was converted into glucose using glucoamylase. Then, the glucose concentration was measured using PGM. The concentration of HBsAg was calculated based on the linear relation between the concentrations of HBsAg and glucose. Under optimal conditions, this assay showed detection limit values of 0.3 to 0.4 ng ml(-1) for "ay" and "ad" subtypes of HBsAg, respectively. The results indicate that the designed assay is comparable to the commercial kits in terms of sensitivity, on-site, specificity, cost, simplicity, portability and reproducibility. The presented method can be used in disadvantaged areas of the world and blood transfusion centers. To the best of our knowledge, this is the first report of using PGMs for HBSAg detection.
机译：乙型肝炎病毒（HBV）感染是主要的公共卫生问题全世界死亡原因。这种感染最重要的诊断标志物是乙型肝炎表面抗原（HBsAg）。在本研究中，设计并研究了一种新颖，廉价，便携式和敏感的ELISA方法，用于基于官能化Fe3O4和Al2O3纳米颗粒的HBsAg诊断，使用廉价和可接近的个人葡萄糖仪（PGM）检测葡萄糖浓度的策略（PGM ）。使用乙型肝炎抗体来构建ELISA系统，以固定在链霉抗生物素蛋白涂覆的磁性铁氧化物颗粒（S-FE3O4）上作为捕获抗体（AB（1））的HbsAg。另外，针对HBsAg（AB（2））和葡糖淀粉酶的不同表位的另一乙型肝炎抗体固定在Al 2 O 3纳米颗粒上。在通过HBsAg分别在S-Fe 3 O 4和Al 2 O 3 NPS上固定在S-Fe 3 O 4和Al 2 O 3 NPS之间的AB（1）和AB（2）之间形成夹层免疫复合物后，使用葡糖淀粉酶将淀粉转化为葡萄糖。然后，使用PGM测量葡萄糖浓度。基于HBsAg和葡萄糖浓度之间的线性关系来计算HBsAg的浓度。在最佳条件下，该测定分别显示出0.3至0.4ng ml（-1）的检测限值分别为HbsAg的“AY”和“AD”亚型。结果表明，设计的测定与敏感性，现场，特异性，成本，简单性，可移植性和再现性方便的商业套件相当。本方法可用于世界弱势地区和血液输血中心。据我们所知，这是第一个使用PGM进行HBsAg检测的报告。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号