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首页> 外文期刊>Journal of insect biotechnology and sericology >Evidence of defect in fibroin secretion in the posterior silk gland cells of the flc mutant of Bombyx mori.
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Evidence of defect in fibroin secretion in the posterior silk gland cells of the flc mutant of Bombyx mori.

机译：Bombyx Mori的FLC突变体后丝腺细胞中纤维素分泌缺陷的证据。

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摘要

The production and excretion of silk proteins in the silk gland cells are very marked processes at the final larval instar of the silkworm, Bombyx mori. Here, proteins in the posterior silk gland tissues and lumen contents in the mutant called flimsy cocoon (flc), wherein fibroin is hardly secreted into the lumen, were separately analyzed by two-dimensional gel electrophoresis. It was shown that the putative small subunit fibroin was accumulated in the cells, and that this protein did not appear in the lumen. In the normal silkworm, this protein was scarce in the tissues but rich in the lumen. Radioactivity counting in the hot-acid insoluble fractions after intraperitoneal injection of [3H]glycine also exhibited a rapid incorporation of label into the posterior silk gland tissues both in the normal and mutant larvae, but differential label was found in the lumen, being very small in the mutant. These results support the idea that the secretion machinery in the flc silk gland cells is deficient..
机译：丝囊细胞中的丝蛋白的生产和排泄是在家蚕的最终幼虫Instar的过程中非常明显的过程，Bombyx Mori。这里，后丝腺组织中的蛋白质和称为脆弱茧（FLC）的突变体中的腔内容物，其中肌蛋白几乎不分泌到腔中，通过二维凝胶电泳分别分析。结果表明，推定的小亚基肌蛋白积聚在细胞中，并且该蛋白质未出现在腔内。在正常的家蚕中，这种蛋白质在组织中稀缺，但富含腔。在腹膜内注射[3H]甘氨酸后，在腹膜内注射后的热酸不溶性级分中的放射性也表现出正常和突变幼虫的后丝腺组织的快速掺入，但在腔内发现了差异标记，非常小在突变体中。这些结果支持FLC丝囊细胞中分泌机械的想法是缺陷的。

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