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首页> 外文期刊>Biotechnology Progress >Enhanced Interferon-β Production by CHO Cells Through Elevated Osmolality and Reduced Culture Temperature
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Enhanced Interferon-β Production by CHO Cells Through Elevated Osmolality and Reduced Culture Temperature

机译：通过提高渗透压和降低培养温度，CHO细胞增强干扰素-β的产生

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摘要

For efficient production of native interferon-β (IFN-β) in recombinant CHO cell culture, the IFN-β molecular aggregation that occurs during culture needs to be minimized. To do so, we investigated the effect of hyperosmolality and hypothermia on IFN-β production and molecular aggregation in rCHO cell culture. Both hyperosmolality (470 mOsm/kg) and hypothermia (32°C) increased specific native INF-β productivity q_(IFN-β) Furthermore, they decreased the IFN-β molecular aggregation, although severe IFN-β molecular aggregation could not be avoided in the later phase of culture. To overcome growth suppression at hyperosmolality and hypothermia, cells were cultivated in a biphasic mode. Cells were first cultivated at 310 mOsmlkg and 37°C for 2 days to rapidly obtain a reasonably high cell concentration. The temperature and osmolality were then shifted to 32°C and 470 mOsmlkg, respectively, to achieve high q_(IFN-β) and reduced IFN-β molecular aggregation. Due to the enhanced q_(IFN-β) and delayed molecular aggregation, the highest native IFN-β concentration achieved on day 6 was 18.03 ± 0.61 mg/L, which was 5.30-fold higher than that in a control batch culture (310 mOsmlkg and 37°C). Taken together, a combination of hyperosmolality and hypothermia in a biphasic culture is a useful strategy for improved native IFN-β production from rCHO cells.
机译：为了在重组CHO细胞培养中有效产生天然干扰素-β（IFN-β），需要将在培养过程中发生的IFN-β分子聚集降至最低。为此，我们研究了高渗透压和低温对rCHO细胞培养物中IFN-β产生和分子聚集的影响。高渗（470 mOsm / kg）和体温过低（32°C）均提高了特定的天然INF-β生产率q_（IFN-β）。此外，尽管无法避免严重的IFN-β分子聚集，但它们降低了IFN-β分子聚集。在文化的后期。为了克服高渗和低温下的生长抑制，以双相模式培养细胞。首先在310 mOsmlkg和37°C下培养细胞2天，以快速获得合理的高细胞浓度。然后将温度和重量克分子渗透压分别转移到32°C和470 mOsmlkg，以实现高q_（IFN-β）和降低的IFN-β分子聚集。由于q_（IFN-β）增强和分子聚集延迟，第6天达到的最高天然IFN-β浓度为18.03±0.61 mg / L，比对照分批培养（310 mOsmlkg）高5.30倍和37°C）。综上所述，双相培养中高渗和低温的组合是改善rCHO细胞天然IFN-β产生的有用策略。

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