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首页> 外文期刊>Journal of Korean Forest Society >DNA Fingerprinting Analysis of Natural Monument Gingko Trees Using Microsatellite Markers
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DNA Fingerprinting Analysis of Natural Monument Gingko Trees Using Microsatellite Markers

机译：使用微卫星标记的自然纪念碑Gingko树的DNA指纹图谱分析

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摘要

This study describes DNA fingerprinting analysis of twenty-three natural monument individuals of Ginkgo biloba using eight microsatellite markers. The average number of observed alleles was 6.875, and the expected heterozygosity and the observed heterozygosity were 0.711 and 0.710, respectively. This results were similar to those of the previous studies on Ginkgo trees analyzed by same markers in China and Japan. PIC value and PD were calculated at 0.677 and 0.9999 respectively, indicating a high individual identification efficiency. In fact, all of the natural monument ginkgo trees and additionally analyzed thirteen general ginkgo tress were identified by genotype comparison. PI and PD calculated in three markers (Ging06, Gb60, Gb61) with the highest PIC values calculated in natural monument ginkgo trees were 8.045* 10"5 and 99.99%, respectively. Thus, these three markers could be preferentially used in DNA fingerprinting for identifying ginkgo tree individuals. The results in this study will be useful for management of natural monument ginkgo trees, proliferation of their progeny and genetic identification of individuals selected in breeding process.
机译：本研究描述了使用八个微卫星标记的二十三只天然纪念碑个体的DNA指纹识别分析。观察等位基因的平均数量为6.875，并且预期的杂合性和观察到的杂合性分别为0.711和0.710。这种结果与以前关于中国和日本的相同标记分析的银杏树研究的结果类似。 PIC值和PD分别在0.677和0.9999以0.677和0.9999计算，表明具有高的个体识别效率。事实上，通过基因型比较鉴定了所有自然纪念碑银杏树和另外分析了十三个一般银杏树。在三个标记（Ging06，GB60，GB61）中计算的PI和PD，分别在天然纪念碑银杏树中计算的最高照片值分别为8.045 * 10“5和99.99％。因此，这三个标记物可以优先用于DNA指纹识别识别银杏树的人。本研究的结果将对自然纪念碑银杏树的管理有用，其后代和遗传鉴定在育种过程中选择的个体的遗传鉴定。

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