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首页> 外文期刊>Journal of mass spectrometry: JMS >Imaging mass spectrometry of gold nanoparticles in a tissue section as an immunohistochemical staining mass probe
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Imaging mass spectrometry of gold nanoparticles in a tissue section as an immunohistochemical staining mass probe

机译：作为免疫组织化学染色探针组织切片中金纳米颗粒的成像质谱

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摘要

For analysis of low abundance peptides in a tissue section, immunohistochemical staining through antibody-antigen interaction is a usual technique. The antibody is conjugated with a probe moiety that aids in highly sensitive detection. Gold nanoparticles, which show excellent chemical stability and variation of surface modifications, are expected to act as a sensitive mass probe to desorb gold ions (Au+, Au-2(+), Au-3(+)) that are distinguishable from fragment ions from organic molecules. Here, green fluorescent proteins (GFP) in a tissue section of a transgenic zebrafish were detected by the gold mass probe conjugated with antibodies. Due to the efficient ionization and desorption of gold ions, imaging mass spectrometry of Au-2(+) ions indicated the distribution of gold nanoparticles stained in a tissue section, and the mass signal distribution was consistent with the area where the GFP-expressing cells were distributed. Conventional immunofluorescence techniques showed intense autofluorescence that come from intrinsic fluorophores in the tissue section. In contrast, the gold nanoparticles acted as an immunostaining mass probe that displayed significantly lower background signals.
机译：为了分析组织切片中的低丰度肽，通过抗体 - 抗原相互作用的免疫组织化学染色是一种常用的技术。抗体与探针部分缀合，探针部分有助于高敏感的检测。具有优异的化学稳定性和表面修饰的变化的金纳米颗粒预期用作敏感的质量探针以解吸金离子（Au +，Au-2（+），Au-3（+）），其与片段离子区分开来自有机分子。这里，通过与抗体缀合的金质量探针检测转基因斑马鱼的组织切片中的绿色荧光蛋白（GFP）。由于金离子的有效电离和解吸，Au-2（+）离子的成像质谱表示染色在组织切片中的金纳米颗粒的分布，并且质量信号分布与表达GFP的细胞的区域一致分布式。常规免疫荧光技术显示出来自组织切片中的内在荧光团的激烈的自发荧光。相反，金纳米颗粒作用为免疫染色质量探针，显示显着更低的背景信号。

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