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首页> 外文期刊>Biotechnology Progress >Accelerating Sample Preparation Through Enzyme-Assisted Microfiltration of Salmonella in Chicken Extract
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Accelerating Sample Preparation Through Enzyme-Assisted Microfiltration of Salmonella in Chicken Extract

机译：酶辅助微滤鸡提取物中沙门氏菌加速样品制备

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Microfiltration of chicken extracts has the potential to significantly decrease the time required to detect Salmonella, as long as the extract can be efficiently filtered and the pathogenic microorganisms kept in a viable state during this process. We present conditions that enable microfiltration by adding endopeptidase from Bacillus amyloliquefaciens to chicken extracts or chicken rinse, prior to microfiltration with fluid flow on both retentate and permeate sides of 0.2 mu m cutoff polysulfone and polyethersulfone hollow fiber membranes. After treatment with this protease, the distribution of micron, submicron, and nanometer particles in chicken extracts changes so that the size of the remaining particles corresponds to 0.4-1 mu m. Together with alteration of dissolved proteins, this change helps to explain how membrane fouling might be minimized because the potential foulants are significantly smaller or larger than the membrane pore size. At the same time, we found that the presence of protein protects Salmonella from protease action, thus maintaining cell viability. Concentration and recovery of 1-10 CFU Salmonella/mL from 400 mL chicken rinse is possible in less than 4 h, with the microfiltration step requiring less than 25 min at fluxes of 0.028-0.32 mL/cm(2) min. The entire procedurefrom sample processing to detection by polymerase chain reactionis completed in 8 h. (C) 2015 American Institute of Chemical Engineers
机译：鸡提取物的微滤具有显着减少检测沙门氏菌所需时间的潜力，只要在此过程中可以有效地过滤提取物并使病原微生物保持存活状态即可。我们提出了通过将解淀粉芽孢杆菌的内肽酶添加到鸡提取物或鸡漂洗液中来进行微滤的条件，然后在截留物和渗透物两面都截留了0.2微米聚砜和聚醚砜中空纤维膜的截留液和渗透液上进行微滤。用这种蛋白酶处理后，鸡提取物中微米，亚微米和纳米颗粒的分布发生变化，因此剩余颗粒的大小相当于0.4-1微米。与溶解的蛋白质的变化一起，这种变化有助于解释如何将膜污染最小化，因为潜在的污染物质明显小于或大于膜的孔径。同时，我们发现蛋白质的存在可保护沙门氏菌免受蛋白酶作用，从而维持细胞活力。可以在不到4小时的时间内从400 mL鸡漂汁中浓缩和回收1-10 CFU沙门氏菌/ mL，而微滤步骤所需的通量为0.028-0.32 mL / cm（2）min则不到25分钟。从样品处理到通过聚合酶链反应检测的整个过程均在8小时内完成。 （C）2015美国化学工程师学会

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