A Transgenic MMTV-Flippase Mouse Line for Molecular Engineering in Mammary Gland and Breast Cancer Mouse Models

Luond Fabiana; Bill Ruben; Vettiger Andrea; Oller Heide; Pelczar Pawel; Christofori Gerhard

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A Transgenic MMTV-Flippase Mouse Line for Molecular Engineering in Mammary Gland and Breast Cancer Mouse Models

机译：用于乳腺和乳腺癌小鼠模型的分子工程转基因MMTV-唾液鼠标

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Genetically engineered mouse models have become an indispensable tool for breast cancer research. Combination of multiple site-specific recombination systems such as Cre/loxP and Flippase (Flp)/Frt allows for engineering of sophisticated, multi-layered conditional mouse models. Here, we report the generation and characterization of a novel transgenic mouse line expressing a mouse codon-optimized Flp under the control of the mouse mammary tumor virus (MMTV) promoter. These mice show robust Flp-mediated recombination in luminal mammary gland and breast cancer cells but no Flp activity in non-mammary tissues, with the exception of limited activity in salivary glands. These mice provide a unique tool for studying mammary gland biology and carcinogenesis in mice.

机译：基因工程的小鼠模型已成为乳腺癌研究的不可缺少的工具。多站点特异性重组系统（如CRE / LOXP和FLIPPASE）/ FRT的组合允许工程到复杂的多层条件小鼠模型。在此，我们报告了在小鼠乳腺肿瘤病毒（MMTV）启动子的控制下表达表达鼠标密码子优化FLP的新型转基因小鼠线的产生和表征。这些小鼠在腔乳腺和乳腺癌细胞中显示出鲁棒的FLP介导的重组，但在非乳腺组织中没有FLP活性，除了唾液腺活性有限。这些小鼠提供了在小鼠中研究乳腺生物学和致癌作用的独特工具。

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来源
《Journal of mammary gland biology and neoplasia》 |2019年第1期|共7页
作者
Luond Fabiana; Bill Ruben; Vettiger Andrea; Oller Heide; Pelczar Pawel; Christofori Gerhard;
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作者单位

Univ Basel Dept Biomed Mattenstr 28 CH-4058 Basel Switzerland;

Univ Basel Dept Biomed Mattenstr 28 CH-4058 Basel Switzerland;

Univ Basel Dept Biomed Mattenstr 28 CH-4058 Basel Switzerland;

Univ Basel Ctr Transgen Models Mattenstr 22 CH-4058 Basel Switzerland;

Univ Basel Ctr Transgen Models Mattenstr 22 CH-4058 Basel Switzerland;

Univ Basel Dept Biomed Mattenstr 28 CH-4058 Basel Switzerland;

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原文格式 PDF
正文语种 eng
中图分类肿瘤学;
关键词
Breast cancer; Flippase; Lineage tracing; Mammary gland; MMTV promoter; Transgenic mice;

机译：乳腺癌;鳞片状;谱系追踪;乳腺;mmtv启动子;转基因小鼠;

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专利

1. A Transgenic MMTV-Flippase Mouse Line for Molecular Engineering in Mammary Gland and Breast Cancer Mouse Models [J] . Luond Fabiana, Bill Ruben, Vettiger Andrea, Journal of mammary gland biology and neoplasia . 2019,第1期

机译：用于乳腺和乳腺癌小鼠模型的分子工程转基因MMTV-唾液鼠标
2. The hyperplastic phenotype in pr-a and pr-b transgenic mice. lessons on the role of estrogen and progesterone receptors in the mouse mammary gland and breast cancer [J] . SampayoR., RecouvreuxS., SimianM. Vitamins and hormones. . 2013,第Null期

机译：pr-a和pr-b转基因小鼠的增生表型。雌激素和孕激素受体在小鼠乳腺和乳腺癌中的作用的经验教训
3. CCL2-driven inflammation increases mammary gland stromal density and cancer susceptibility in a transgenic mouse model [J] . Xuan Sun, Danielle J. Glynn, Leigh J. Hodson, Breast Cancer Research . 2017,第1期

机译：CCL2驱动的炎症增加了转基因小鼠模型中的乳腺基质密度和癌症易感性
4. 3D optoacoustic tomography: From molecular targets in mouse models to functional imaging of breast cancer [C] . Oraevsky Alexander Conference on Lasers and Electro-Optics . 2014

机译：3D光声层析成像：从小鼠模型中的分子靶点到乳腺癌的功能成像
5. The role of the Pea3 subfamily ets genes in mammary tumorigenesis: Use of transgenic mouse models of breast cancer [D] . Shepherd, Trevor Grey 2002

机译：Pea3亚家族ets基因在乳腺肿瘤发生中的作用：乳腺癌的转基因小鼠模型的使用
6. Transcriptomic signature of Bexarotene (Rexinoid LGD1069) on mammary gland from three transgenic mouse mammary cancer models [O] . Martin C Abba, Yuhui Hu, Carla C Levy, 2008

机译：贝沙罗丁（Rexinoid LGD1069）在三种转基因小鼠乳癌模型中的乳腺上的转录组学特征
7. Transcriptomic signature of Bexarotene (Rexinoid LGD1069) on mammary gland from three transgenic mouse mammary cancer models [O] . 2008

机译：贝沙罗丁（Rexinoid LGD1069）在三种转基因小鼠乳癌模型中的乳腺上的转录组学特征
8. Use of a Transgenic Mouse Model With a Regulatable Estrogen Receptor Alpha (ER) to Study the Role of ER in Mammary Gland Development and Cancer [R] . Sugg, S. L. 2004

机译：使用具有可调节的雌激素受体α（ER）的转基因小鼠模型来研究ER在乳腺发育和癌症中的作用

A Transgenic MMTV-Flippase Mouse Line for Molecular Engineering in Mammary Gland and Breast Cancer Mouse Models

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