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High Production of D-Tagatose by the Addition of Boric Acid

机译:加入硼酸可高产D-塔格糖

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摘要

An L-arabinose isomerase mutant enzyme from Geobacillus thermodenitrificans was used to catalyze the isomerization of D-galactose to D-tagatose with boric acid.Maximum production of D-tagatose occurred at pH 8.5-9.0,60 °C,and 0.4 molar ratio of boric acid to D-galactose,and the production increased with increasing enzyme concentration.Under the optimum conditions,the enzyme (10.8 units/mL) converted 300 g/L D-galactose to 230 g/L D-tagatose for 20 h with a yield of 77% (w/w);the production and conversion yield with boric acid were 1.5-fold and 24% higher than without boric acid,respectively.In 24 h,the enzyme produced 370 g/L D-tagatose from 500 g/L D-galactose with boric acid,corresponding to a conversion yield of 74% (w/w) and a production rate of 15.4 g/L-h.The production and yield of D-tagatose obtained in this study are unprecedented.
机译:用热酸革兰氏芽孢杆菌的L-阿拉伯糖异构酶突变酶催化硼酸将D-半乳糖异构化为D-塔格糖,在8.5-9.0,60°C的pH值为0.4的条件下,D-塔格糖的产量最高。在最佳条件下,酶(10.8单位/毫升)将300 g / L D-半乳糖转化为230 g / L D-塔格糖,历时20 h。产率为77%(w / w);使用硼酸的产量和转化率分别比不使用硼酸高1.5倍和24%。在24小时内,酶从500克中产生370克/升D-塔格糖/ L D-半乳糖与硼酸对应,转化率为74%(w / w),生产率为15.4 g / Lh。本研究中获得的D-塔格糖的产量和产率是前所未有的。

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