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首页> 外文期刊>Biotechnology Progress >Single-Bead-Based Immunofluorescence Assay for Snake Venom Detection
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Single-Bead-Based Immunofluorescence Assay for Snake Venom Detection

机译：基于单珠的免疫荧光检测蛇毒

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In this communication,we reported a rapid and sensitive immunofluorescence method for the detection of snake venom by using microscale polystyrene beads as platform combined with semiconductor quantum dots(Qdots)as fluorescence label.Briefly,control rabbit IgG or capture antibody for venom was covalently immobilized onto the microspheres(surface activated with carboxyl group,dyed with different color)to form the control or capture beads.When incubated with the testing samples,the venom binds to the specific capture beads to form the complex through antibody-antigen interaction.Then,the second antibody conjugated Qdot was added,which targeted the Qdot to bind to the capture bead/antigen complex.The complex can be directly observed under a UV microscope.The system was applied to the testing of Naja kaouthia venom.Fluorescent microscopic images of QD-labeled capture beads demonstrated that QD-antibody conjugates could evenly and completely attach to the surface of capture beads,indicating that the conjugated antibody molecules remained active and were able to recognize their specific target in solution.The detection limit of this method was 5-10 ng/mL.The detection could be completed within 3 h.
机译：在本次交流中，我们报道了一种快速灵敏的免疫荧光方法，该方法使用微型聚苯乙烯珠作为平台并结合半导体量子点（Qdots）作为荧光标记来检测蛇毒。简而言之，共价固定了针对兔毒的对照兔IgG或捕获抗体到微球上（被羧基活化的表面，染成不同的颜色）形成对照或捕获珠。当与测试样品一起孵育时，毒液通过抗体-抗原相互作用与特异性捕获珠结合形成复合物。加入第二个结合Qdot的抗体，该Qdot靶向Qdot与捕获珠/抗原复合物结合。该复合物可以在紫外显微镜下直接观察到。该系统用于检测眼镜蛇毒液.QD的荧光显微图像标记的捕获珠表明，QD抗体结合物可以均匀且完全地附着在捕获珠的表面，表明该方法的检测限为5-10 ng / mL，可在3 h内完成检测。

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