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首页> 外文期刊>Biotechnology Progress >Corecovery of Lipids and Fermentable Sugars from Rhodosporidium toruloides Using Ionic Liquid Cosolvents: Application of Recycle to Batch Fermentation
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Corecovery of Lipids and Fermentable Sugars from Rhodosporidium toruloides Using Ionic Liquid Cosolvents: Application of Recycle to Batch Fermentation

机译：使用离子液体助溶剂从球形假单胞菌中脂类和可发酵糖的岩心发现：回收在间歇发酵中的应用

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摘要

This work evaluates the ability of an ionic liquid-methanol cosolvent system to extract lipids and recycle fermentable sugars recovered from oil-bearing Rhodosporidium toruloides grown in batch culture on defined media using glucose and xylose as carbon sources. Growth on the recycled mixed carbon substrate was successful with glucose consumed before xylose and overall cell mass to lipid yields (Y_(P/X)) between 57% and 61% (w/w relative to whole dried cell mass) achieved. Enzymatic hydrolysis of the delipified carbohydrate fraction recovered approximately 9%-11% (w/w) of the whole dried cell mass as fermentable sugars, which were successfully recycled as carbon sources without further purification. In total, up to 70% (w/w) of the whole dried cell mass was recovered as lipids and fermentable sugars and the substrate to lipid yields (Y_(P/S)) was increased from 0.12 to 0.16 g lipid/g carbohydrate consumed, highlighting the promise of this approach to process lipid bearing cell biomass.
机译：这项工作评估了离子液体-甲醇助溶剂系统提取脂质和回收可发酵糖的能力，这些糖是从在葡萄糖和木糖作为碳源的限定培养基上分批培养的含油罗氏假单胞菌中提取的。在木糖和总细胞质量至脂质产量（Y_（P / X））达到57％至61％（w / w相对于整个干燥细胞质量）之前，消耗了葡萄糖就可以成功地在回收的混合碳底物上生长葡萄糖。脱脂碳水化合物馏分的酶促水解回收了整个干细胞团中约9％-11％（w / w）的可发酵糖，可将糖成功地循环用作碳源，而无需进一步纯化。总计，高达70％（w / w）的干细胞总量被回收为脂质和可发酵糖，底物脂质产量（Y_（P / S））从0.12 g脂质/ g碳水化合物增加到0.16 g消耗，突出了这种方法处理脂质细胞生物质的前景。

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