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首页> 外文期刊>Journal of Plant Biochemistry and Biotechnology >Chitosan effects on the elevation of essential oils and antioxidant activity ofCarum copticumL. seedlings and callus cultures under in vitro salt stress
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Chitosan effects on the elevation of essential oils and antioxidant activity ofCarum copticumL. seedlings and callus cultures under in vitro salt stress

机译：壳聚糖对Copticuml精油和抗氧化活性升高的影响。 在体外盐胁迫下的幼苗和愈伤组织培养物

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Chitosan is a natural nontoxic biopolymer, which is indicated as an aid for reducing oxidative injury caused by salt stress. To explore the potential effects of chitosan on the production of secondary metabolites and antioxidant activity ofCarum copticumL., commonly known as Ajwain, seedlings and callus under in vitro salt stress, total phenolics, protein content, antioxidant enzyme activities, and essential oil contents were measured. Two-week-oldC. copticumwas cultured on MS medium containing 0, 10 and 20 mg L(-1)chitosan and 0 and 100 mM NaCl. Calli induced on MS medium supplemented with 4 mu M benzyl amino purine and 1 mu M 2, 4-dichlorophenoxyacetic acid were cultured on MS medium containing 4 mu M BAP, 1 mu M 2, 4-D, and different concentrations of chitosan with 100 mM NaCl. The total phenolics, protein content, and antioxidant enzyme activities significantly increased in callus cultures and seedlings under NaCl treatment, except for the root phenolic content. On the other hand, chitosan suppressed phenolic accumulation and antioxidant enzyme activities under NaCl treatment. Furthermore, both NaCl and chitosan enhanced the contents of thymol andp-cymene in seedlings and calli. No significant difference was observed between calli and seedlings. Regarding the effects of chitosan, 20 mg L(-1)chitosan found to be most effective on phenol content with increased production of thymol andp-cymene, and it could serve as a favorable excipient for the pharmaceutical industry. These results suggest that plant tissue culture techniques are a promising approach for improving the production of target secondary metabolites through abiotic elicitors supplemented to the medium.
机译：壳聚糖是一种天然的无毒生物聚合物，其表示为减少由盐胁迫引起的氧化损伤的辅助剂。探讨壳聚糖对Copticuml的次级代谢物和抗氧化活性的潜在影响。，通常称为体外盐胁迫下的Ajwain，幼苗和愈伤组织，总酚类，蛋白质含量，抗氧化酶活性和精油内容物。为期两周的老人。 Copticumwas在含有0,10和20mg L（-1）壳聚糖和0和100mM NaCl的MS培养基上培养。在含有4μm苄基氨基嘌呤和1μm2，4-二氯苯氧基乙酸的MS培养基上诱导的Calli在含有4μmbap，1μm2,4-d和100μm的不同壳聚糖的MS培养基上培养。 mm nacl。除了根酚类含量外，愈伤组织培养物和幼苗的总酚类，蛋白质含量和抗氧化酶活性显着增加。另一方面，壳聚糖在NaCl处理下抑制酚类积聚和抗氧化酶活性。此外，NaCl和壳聚糖均增强了幼苗和愈伤组织中养血管和p-cymene的含量。在Calli和幼苗之间观察到没有显着差异。关于壳聚糖的作用，20mg L（-1）壳聚糖发现对苯酚含量最有效的苯酚含量增加，并且它可以作为制药行业的有利赋形剂。这些结果表明，植物组织培养技术是通过补充到培养基的非生物菌，改善目标次级代谢产物的有希望的方法。

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