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High-speed broadband Fourier-transform coherent anti-stokes Raman scattering spectral microscopy

机译:高速宽带傅立叶变换相干反斯托克斯拉曼散射光谱显微镜

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摘要

We demonstrate broadband Fourier-transform coherent anti-Stokes Raman scattering (FT-CARS) spectral microscopy with a pixel dwell time of 42 mu s, which is ~50 times shorter than the shortest-to-date pixel dwell time for CARS spectral microscopy. Our broadband FT-CARS spectral microscope is composed of an FT-CARS spectrometer, a rapid galvanometric scanner, and a high-speed image acquisition circuit, enabling a frame rate of 2.4 fps with a pixel resolution of 100 x 100 pixels, a bandwidth of 600-1,200 cm(-1), a spatial resolution of 0.95 mu m, and a spectral resolution of 37 cm(-1). As a proof-of-principle demonstration, we used the high-speed FT-CARS spectral microscope to perform CARS imaging of polymer beads and Haematococcus lacustris cells. Our high-speed broadband CARS spectral microscope holds promise for studying rapid cellular dynamics, such as signaling, cell-to-cell communication, and molecular transport in a label-free manner.
机译:我们展示了宽带傅里叶变换相干反斯托克斯拉曼散射(FT-CARS)光谱显微镜,其像素停留时间为42μs,这比汽车光谱显微镜的最短到日期像素停留时间短约50倍。 我们的宽带FT-CARS谱显微镜由FT车谱仪,快速电压计扫描仪和高速图像采集电路组成,使得帧速率为2.4 FPS,像素分辨率为100 x 100像素,带宽 600-1,200厘米(-1),空间分辨率为0.95μm,光谱分辨率为37厘米(-1)。 作为原则上的证据演示,我们使用高速FT-CARS光谱显微镜进行高分子珠和HaematoCoccus朗物池的血管成像。 我们的高速宽带汽车光谱显微镜具有研究快速蜂窝动态,例如以无标记的方式研究快速蜂窝动态,例如信号传导,细胞通信和分子运输。

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