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首页> 外文期刊>Journal of tissue engineering and regenerative medicine >The efficacy of a novel collagen-gelatin scaffold with basic fibroblast growth factor for the treatment of vocal fold scar
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The efficacy of a novel collagen-gelatin scaffold with basic fibroblast growth factor for the treatment of vocal fold scar

机译：新型胶原蛋白 - 明胶支架与碱性成纤维细胞生长因子治疗声带瘢痕的疗效

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摘要

Vocal fold scar remains a therapeutic challenge. Basic fibroblast growth factor (bFGF) was reported to have regenerative effects for vocal fold scar, although it has the disadvantage of rapid absorption in vivo. A collagen-gelatin sponge (CGS) can compensate for the disadvantage by providing a sustained release system. The current study evaluated the efficacy of CGS combined with bFGF on vocal fold scar, using rat fibroblasts for an in vitro model and a canine in vivo model. We prepared fibroblasts from scarred vocal folds (sVFs) in rats and showed that bFGF accelerated cell proliferation and suppressed expression levels of cleaved caspase 3 and alpha-smooth muscle actin. Has 1, Has 3, Fgf2, Hgf and Vegfa mRNA levels were significantly upregulated, while Col1a1 and Col3a1 were dose-dependently downregulated, with a maximum effect at 100 ng/ml bFGF. In an in vivo assay, 6 weeks after lamina propria stripping, beagles were divided into three groups: CGS alone (CGS group); CGS with bFGF (7 mu g/cm(2); CGS + bFGF group); or a sham-treated group. Vibratory examination revealed that the glottal gap was significantly reduced in the bFGF group and the two implanted groups, whereas the CGS + bFGF group showed higher mucosal wave amplitude. Histological examination revealed significantly restored hyaluronic acid and elastin redistribution in the CGS + bFGF group and reductions in dense collagen deposition. These results provide evidence that CGS and bFGF combination therapy may have therapeutic potential and could be a promising tool for treating vocal fold scar. Copyright (C) 2015 John Wiley & Sons, Ltd.
机译：声带疤痕仍然是治疗性挑战。据报道，基本的成纤维细胞生长因子（BFGF）对声带疤痕具有再生效果，尽管它具有体内快速吸收的缺点。胶原蛋白 - 明胶海绵（CGS）可以通过提供持续释放系统来补偿缺点。目前的研究评估了CGS与体外模型中的大鼠成纤维细胞和体内模型中的犬类相结合的CGS与声带折痕瘢痕的疗效。我们在大鼠中从疤痕的声带（SVF）中制备了成纤维细胞，并表明BFGF加速细胞增殖和抑制的裂解胱天蛋白酶3和α-平滑肌肌动蛋白的表达水平。具有1，具有3，FGF2，HGF和VEGFA mRNA水平显着上调，而COL1A1和COL3A1被剂量依赖性下调，最大效果在100ng / mL BFGF下。在体内测定中，6周后椎板普形剥离后，小珠分为三组：单独CGS（CGS组）; CGS具有BFGF（7μg/ cm（2）; cgs + bfgf组）;或假治疗组。振动检查表明，BFGF组和两个植入基团中显着降低了光泽间隙，而CGS + BFGF组显示出较高的粘膜波振幅。组织学检查显示CGS + BFGF组中的显着恢复透明质酸和弹性蛋白再分配，并降低致密胶原沉积。这些结果提供了证据表明CGS和BFGF组合治疗可能具有治疗潜力，并且可能是治疗声带疤痕的有希望的工具。版权所有（c）2015 John Wiley＆Sons，Ltd。

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