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首页> 外文期刊>American Journal of Microbiology >IDENTIFICATION AND CHARACTERIZATION OF ANTIBIOTIC-PRODUCING ACTINOMYCETES ISOLATES
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IDENTIFICATION AND CHARACTERIZATION OF ANTIBIOTIC-PRODUCING ACTINOMYCETES ISOLATES

机译：产生抗生素的放线菌分离株的鉴定和鉴定

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Actinomycetes are a gram-positive, filamentous subgroup of bacteria most known for antibiotic production. In fact, most of the antibiotics available today have originated from actinomycetes, namely from the genus Streptomyces. Novel bacteria with antimicrobial activities have been discovered from bacterial screen studies for decades and there is still much more yet to be unearthed. One hundred seventy five strains of actinomycetes were isolated from 38 different soil samples from different locations in Patna, India. Potential antibiotic producers were screened against four test microorganisms (Escherichia coli MTCC 739, Staphylococcus aureus MTCC 96, Streptomyces lividans TK23 MTCC 4 and Candida albicans MTCC 227). The bioassay revealed that about 26% of actinomycetes isolates were active against at least one of the test microorganism. Characterization of a selected isolate has led to identification of a unique strain of actinomycetes (MP 525) showing broad-spectrum antibacterial and antifungal properties. The strain MP 525 has been morphologically characterized as Streptomyces sp. and deposited at MTCC, Chandigarh, India with accession number 8723. The 16S rRNA gene of the strain Streptomyces sp. US7 MTCC 8723 was sequenced and the DNA sequence was deposited at NCBI, Bethesda (GenBank accession. No. HQ659005). On the basis of λmax values of culture filtrates, it has been suggested that the strain might be producing LL-E19085-like antibacterial and a flavone glycoside-like antifungal antibiotics, which can be further exploited for industrial and biological applications.
机译：放线菌是细菌最常见的革兰氏阳性丝状亚组。实际上，当今可获得的大多数抗生素都起源于放线菌，即链霉菌属。数十年来，从细菌筛选研究中发现了具有抗菌活性的新型细菌，并且还有更多的东西待发掘。从印度巴特那不同地区的38个不同土壤样品中分离出175个放线菌菌株。针对四种测试微生物（大肠杆菌MTCC 739，金黄色葡萄球菌MTCC 96，淡链霉菌链霉菌TK23 MTCC 4和白色念珠菌MTCC 227）筛选潜在的抗生素生产者。生物测定表明，约26％的放线菌分离物对至少一种测试微生物具有活性。所选分离物的表征已导致鉴定出唯一的放线菌菌株（MP 525），该菌株显示出广谱的抗菌和抗真菌特性。 MP 525菌株在形态上已被表征为链霉菌。并保藏在印度昌迪加尔的MTCC，保藏号为8723。链霉菌（Streptomyces sp）菌株的16S rRNA基因。对US7 MTCC 8723进行测序，并将DNA序列保藏在Bethesda的NCBI（GenBank登录号：HQ659005）。根据培养滤液的λmax值，已表明该菌株可能产生LL-E19085样抗菌剂和黄酮糖苷样抗真菌抗生素，可进一步用于工业和生物学应用。

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