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首页> 外文期刊>Journal of the Taiwan Institute of Chemical Engineers >Trehalose production via merged secretion, purification, and immobilization of trehalose synthase in Bacillus subtilis
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Trehalose production via merged secretion, purification, and immobilization of trehalose synthase in Bacillus subtilis

机译：通过合并的分泌物，纯化和芽孢杆菌合成酶的分泌物，纯化和固定的产量生产

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摘要

Here, we established a one-step reaction method using trehalose synthase to produce trehalose from maltose. Bacillus subtilis was used as an expression host, and the various B. subtilis signal peptides and chitin-binding domains (ChBD) from Bacillus circulans WL-12 chitinase A1 were fused with the N- and C-termini, respectively, of trehalose synthase from the Picrophilus torridus DSM 9790 (PTTS) gene. Results showed that trehalose synthase was secreted by the YwbN signal peptide of B. subtilis and bound specifically to the chitin beads. The conversion yield of the immobilized enzyme was up to 69.07%, which was higher than that of the free enzyme (65.40%). Furthermore, the immobilized enzyme also retained 50% of its residual activity after the 21st repeated use. The chitin beads maintained 55% adsorption capacity after being reused 53 times. The results indicate the potential usefulness of the developed approach for trehalose production. (C) 2017 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
机译：在这里，我们建立了使用海藻糖合成酶的一步反应方法，从麦芽糖中产生海藻糖。使用枯草芽孢杆菌作为表达宿主，分别与枯草芽孢杆菌WL-12丁质酶A1的各种B.枯草芽孢杆菌信号肽和丁蛋白结合结构酶A1分别与N-和C-末端的海藻糖合成酶融合微微嗜菌托里达斯DSM 9790（PTTS）基因。结果表明，海藻糖合成酶被B.枯草芽孢杆菌的YWBN信号肽分泌并与丁蛋白珠粒结合。固定化酶的转化率高至69.07％，高于游离酶（65.40％）。此外，在第21次重复使用后，固定化酶在21秒后，还保留了其残余活性的50％。在重复使用53次后，甲壳素珠粒保持55％的吸附能力。结果表明了对海藻糖生产的发达方法的潜在有用性。 （c）2017台湾化工工程师研究所。 elsevier b.v出版。保留所有权利。

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