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首页> 外文期刊>Journal of the Association for Research in Otolaryngology: JARO >Enhanced survival of spiral ganglion cells after cessation of treatment with brain-derived neurotrophic factor in deafened guinea pigs.
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Enhanced survival of spiral ganglion cells after cessation of treatment with brain-derived neurotrophic factor in deafened guinea pigs.

机译：在聋豚鼠脑源性神经营养因子治疗后增强螺旋神经节细胞的存活。

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摘要

Exogenous delivery of neurotrophic factors into the cochlea of deafened animals rescues spiral ganglion cells (SGCs) from degeneration. To be clinically relevant for human cochlear implant candidates, the protective effect of neurotrophins should persist after cessation of treatment and the treated SGCs should remain functional. In this study, the survival and functionality of SGCs were investigated after temporary treatment with brain-derived neurotrophic factor (BDNF). Guinea pigs in the experimental group were deafened, and 2 weeks later, the right cochleae were implanted with an electrode array and drug delivery cannula. BDNF was administered to the implanted cochleae during a 4-week period via a mini-osmotic pump. After completion of the treatment, the osmotic pumps were removed. Two weeks later, the animals were killed and the survival of SGCs was analyzed. To monitor the functionality of the auditory nerve, electrically evoked auditory brainstem responses (eABRs) were recorded in awake animals throughout the experiment. BDNF treatment resulted in enhanced survival of SGCs 2 weeks after cessation of the treatment and prevented the decreases in size and circularity that are seen in the untreated contralateral cochleae. The amplitude of the suprathreshold eABR response in BDNF-treated animals was significantly larger than in deafened control animals and comparable to that in normal-hearing control animals. The amplitude in the BDNF-treated group did not decrease significantly after cessation of treatment. The eABR latency in BDNF-treated animals was longer than normal and comparable to that in deafened control animals. These morphological and functional findings demonstrate that neurotrophic intervention had a lasting effect, which is promising for future clinical application of neurotrophic factors in implanted human cochleae.
机译：将神经营养因素的外源性递送到聋动物的耳蜗中，从退化中救出螺旋神经节细胞（SGC）。为了临床相关的人耳蜗植入候选者，神经营养素的保护作用应该在停止治疗后持续存在，并且治疗的SGCs应保持功能。在该研究中，用脑衍生的神经营养因子（BDNF）进行临时治疗后研究了SGCs的存活率和功能。实验组中的豚鼠叫醒，并2周后，用电极阵列和药物输送套管注入右耳蜗。通过迷你渗透泵在4周的时间内给予植入的耳蜗施用BDNF。完成处理后，除去渗透泵。两周后，分析了动物的生存，分析了SGC的存活。为了监测听觉神经的功能，在整个实验中，唤醒动物记录了电诱发的听觉脑干响应（EABRS）。 BDNF治疗导致停止治疗后2周的SGCs存活率，并阻止在未经处理的对侧耳蜗中看到的大小和圆形的降低。 BDNF处理动物中Suprathreshold EABR反应的幅度显着大于聋人对照动物中的响应，并与正常听力控制动物相当。在停止治疗后，BDNF处理组中的振幅不会显着降低。 BDNF处理的动物的EABR潜伏期比正常的正常性较长，并且与聋人对照动物的相当。这些形态和功能性研究结果表明，神经营养干预具有持久的效果，这对未来的神经营养因子在植入的人耳蜗中的临床应用是有前途的。

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