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首页> 外文期刊>Biotechnology Progress >Production of 2,3-Butanediol by Klebsiella Oxytoca From Various Sugars in Microalgal Hydrolysate
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Production of 2,3-Butanediol by Klebsiella Oxytoca From Various Sugars in Microalgal Hydrolysate

机译：氧化克雷伯氏菌在微藻水解物中由各种糖生产2,3-丁二醇

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摘要

A new fermentation process using a mixed sugar medium is proposed in this study for 2,3-butanediol (2,3-BDO) production. The medium contained seven different monosugars known to be present in Nannochloropsis oceanica hydrolysate. The performance of each sugar when existing alone or together with glucose was evaluated. All the sugars except fucose were successfully metabolized for 2,3-BDO production. A 2,3-BDO yield of 0.31g/g was achieved with the mixed sugar medium, which was very close to that with the glucose-only medium. However, the 2,3-BDO productivity (0.28 g L-1 h(-1)) was found to be about 30% lower than that with glucose, implying, as expected, the existence of glucose repression on the uptake of other sugars. Strain development is in need to remove such negative effect of glucose for improved process efficiency. Fucose with the lowest uptake rate and no contribution to 2,3-BDO production can be a high value-added byproduct, once recovered and purified. (C) 2015 American Institute of Chemical Engineers
机译：在这项研究中，提出了一种使用混合糖介质的新发酵工艺，用于生产2,3-丁二醇（2,3-BDO）。该培养基包含七种不同的单糖，已知存在于Nannochloropsis oceanica水解物中。评估了每种糖单独存在或与葡萄糖一起存在时的性能。除岩藻糖外，所有糖均已成功代谢产生2,3-BDO。混合糖培养基的2,3-BDO产量为0.31g / g，非常接近纯葡萄糖培养基。然而，发现2,3-BDO的生产率（0.28 g L-1 h（-1））比使用葡萄糖的生产率低约30％，这意味着，正如预期的那样，存在葡萄糖抑制对摄取其他糖的抑制作用。需要菌株研发来消除葡萄糖的这种负面影响，以提高工艺效率。摄取率最低且对2,3-BDO生产无贡献的岩藻糖，一旦被回收和纯化，便可以成为高附加值的副产物。 （C）2015美国化学工程师学会

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