Monitoring of ribosomes and subunits in Escherichia coli during productionof glucose isomerase using flow field-flow fractionation

Nilsson M.; Wahlund KG.; Bulow L.

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Monitoring of ribosomes and subunits in Escherichia coli during productionof glucose isomerase using flow field-flow fractionation

机译：使用流场-流分馏法监测葡萄糖异构酶生产过程中大肠杆菌中的核糖体和亚基

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Ribosomal components and tRNA of E. coli TG1 producing a recombinant enzyme, glucose isomerase, were monitored by asymmetrical flow field-flow fractionation. The growth temperatures were 20, 30 and 37 degrees C. Results indicate a correlation between translational capacity and the amount obtained of the recombinant enzyme. Highest yield was obtained when cultivating at 30 degrees C.

机译：通过不对称流场-流分离来监测产生重组酶葡萄糖异构酶的大肠杆菌TG1的核糖体成分和tRNA。生长温度为20、30和37℃。结果表明翻译能力与重组酶的获得量之间的相关性。在30摄氏度下培养可获得最高产量。

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《Biotechnology Techniques》 |1998年第6期|共4页
作者
Nilsson M.; Wahlund KG.; Bulow L.;
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正文语种 eng
中图分类分子生物学;
关键词
Expression;

机译：表达;

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1. Monitoring of ribosomes and subunits in Escherichia coli during productionof glucose isomerase using flow field-flow fractionation [J] . Nilsson M., Wahlund KG., Bulow L. Biotechnology Techniques . 1998,第6期

机译：使用流场-流分馏法监测葡萄糖异构酶生产过程中大肠杆菌中的核糖体和亚基
2. Expression of Vitreoscilla hemoglobin in Escherichia coli enhancesribosome and tRNA levels: A flow field-flow fractionation study [J] . Nilsson M., Kallio PT., Bailey JE., Biotechnology Progress . 1999,第2期

机译：玻璃体内血红蛋白在大肠杆菌中的表达增强了核糖体和tRNA水平：流场-流分馏研究
3. Bacteria sorting by field-flow fractionation. Application to whole-cell Escherichia coli vaccine strains [J] . Reschiglian P., Zattoni A., Roda B., Analytical chemistry . 2002,第19期

机译：通过场流分离对细菌进行分类。在全细胞大肠杆菌疫苗株中的应用
4. Can Chitosans get sterilized by Electron Irradiation? Monitoring Chitosan Degradation by Flow Field-Flow Fractionation and Multi-Angle Light Scattering [C] . Christian Augsten, Wolfgang Knolle, Karsten Maeder Controlled Release Society Annual Meeting and Exposition . 2007

机译：壳聚糖可以通过电子辐照灭菌吗？通过流场-流分离和多角度光散射监测壳聚糖的降解
5. Escherichia coli RNA helicase DbpA and its role in assembly of the large subunit of the ribosome [D] . Sharpe Elles, Lisa Marie. 2008

机译：大肠杆菌rna helicase dbpa及其在核糖体的大亚基组装中的作用
6. Selection of motile nonchemotactic mutants of Escherichia coli by field-flow fractionation. [O] . H C Berg, L Turner 1991

机译：通过场流分级分离技术筛选大肠杆菌的非趋化性突变体。
7. Selection of motile nonchemotactic mutants of Escherichia coli by field-flow fractionation. [O] . Berg, H C, Turner, L 1991

机译：通过场流分级分离技术筛选大肠杆菌的非趋化性突变体。

Monitoring of ribosomes and subunits in Escherichia coli during productionof glucose isomerase using flow field-flow fractionation

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