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Analysis of the resistance gene in Streptococcus pneumoniae

机译:肺炎链球菌抗性基因分析

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Resistance genes were determinded for 81 strains of Streptococcus pneumoniae isolated from Ehime University hospital, during 2002 and 2003 by various clinical material. In penicillin-binding proteins of mutation, there were 74 strains; pbp2x mutation 23 strains (28.4%), pbp2b mutation one strain (1.2%), pbp1a + pbp2x mutations 5 strains (6.2%), pbp2x + pbp2b mutations 18 strains (22.2%) and all mutations 27 strains (33.3%). As for the result of macrolide resistance genes, there were 67 strains; mefA gene 20 strains (24.7%), ermB gene 46 strains (56.8%) and both gene one strain (1.2%). In the analysis of gyrA gene and parC gene, 3 strains (3.7%) had both gene mutations, and 26 strains (32.1%) had only parC gene mutation. There was more of an increase than before in isolates, two or more mutation strains with PBPs gene, ermB gene holding strains and the levofloxacin resistance strain. These results suggest that the gyrA gene or parC gene mutation strains hold PBPs gene mutation and macrolide resistance genes in a high rate, and there will be more drug resistance in the future.
机译:通过各种临床资料,在2002年和2003年,确定了抗肺炎链球菌肺炎群的81株肺炎链球菌菌株的抗性基因。在青霉素结合蛋白质的突变中,存在74株; PBP2X突变23株(28.4%),PBP2B突变一个菌株(1.2%),PBP1A + PBP2X突变5株(6.2%),PBP2X + PBP2B突变18株(22.2%)和所有突变27株(33.3%)。至于大环内酯抗性基因的结果,存在67株; MEFA基因20株(24.7%),ERMB基因46株(56.8%)和一个菌株(1.2%)。在对Gyra基因和Parc基因分析中,3株(3.7%)具有两个基因突变,26个菌株(32.1%)仅具有Parc基因突变。通常比在分离物,两个或更多个突变菌株中的增加的增加,具有PBPS基因,ERMB基因保持菌株和左氧氟沙星抗性菌株。这些结果表明,Gyra基因或Parc基因突变菌株以高速率保持PBPS基因突变和大环内酯抗性基因,未来将有更多的耐药性。

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